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His Tag antibody

Name: His Tag antibody
SKU: ABIN652278
Price: 429 USD
Availability: InStock

Reactivity: Human WB Host: Rabbit Polyclonal RB19960 unconjugated

All lanes : Anti-His Tag Antibody at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: 293T/17 transfected with 12tag whole cell lysate Lane 3: 12tag recombinant protein Lysates/proteins at 1 μg per lane.

Anti-His Tag at 1:8000 dilution + 12tag recombination protein Lysates/proteins at 20 ng per lane.

2 images

(1 reference)

Catalog No. ABIN652278

Datasheet as PDF

Target See all His Tag Antibodies

His Tag
Reactivity
18

4

4

1

1

1
Human
Host
93

55

12

9

8

7

2

1
Rabbit
Compatible Secondaries
Clonality
106

68

10
Polyclonal
Conjugate
90

22

14

12

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
Application
152

103

38

27

25

17

14

14

14

13

13

7

7

6

6

5

4

4

3

3

2

2

1

1

1

1
Western Blotting (WB)

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

Poly-HIS peptide were used to produced this antibody.

Clone

RB19960

Isotype

Ig Fraction

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Discover our top product His Tag Primary Antibody
anti-His Tag antibody
WB, ELISA, IP, IHC Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody
Reactivity: Human WB Host: Mouse Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA, IP, IHC, FACS Host: Mouse Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS Host: Mouse Monoclonal 6G2A9 unconjugated

anti-His Tag antibody
Reactivity: Tag, Human WB Host: Mouse Monoclonal 2D5 unconjugated

anti-His Tag antibody (HRP)
WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2 HRP

anti-His Tag (C-Term) antibody
WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2 Biotin

anti-His Tag (N-Term) antibody (Biotin)
WB, ELISA, IP, FACS, ICC, IHC (p) Host: Rabbit Monoclonal RM146 Biotin

Application Notes

WB: 1:8000. WB: 1:1000. WB: 1:1000

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Jiang, Zhao, Xiong, Wang, He, Wang, Chen, Geng, Huang, Ouyang, Lai: "Immune responses of channel catfish following the stimulation of three recombinant flagellins of Yersinia ruckeri in vitro and in vivo." in: Developmental and comparative immunology, Vol. 73, pp. 61-71, (2018) (PubMed).
Target

His Tag

Alternative Name

6 X His Tag (His Tag Products)

Target Type

Tag

Background

Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.