His Tag antibody

Catalog No. ABIN652278

Cited in 1 publication. The Rabbit Polyclonal anti-His Tag antibody (Clone RB19960) (ABIN652278) specifically detects His Tag in WB. The antibody is reactive with Human samples.

Quick Overview for His Tag antibody (ABIN652278)

Target: His Tag

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB19960

Product Details anti-His Tag Antibody

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: Poly-HIS peptide were used to produced this antibody.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:8000. WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-His Tag antibody (ABIN652278)

Jiang, Zhao, Xiong, Wang, He, Wang, Chen, Geng, Huang, Ouyang, Lai: "Immune responses of channel catfish following the stimulation of three recombinant flagellins of Yersinia ruckeri in vitro and in vivo." in: Developmental and comparative immunology, Vol. 73, pp. 61-71, (2018) (PubMed).

Target Details for His Tag

Target: His Tag

Alternative Name: 6 X His Tag

Target Type: Tag

Background: Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.