CD14 Molecule (CD14) antibody

Details for Product No. ABIN638430
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Antigen
Reactivity
Human, Non-Human Primate
(368), (68), (29), (23), (22), (20), (15), (12), (10), (8), (8), (8), (7), (7), (1), (1)
Host
Mouse
(328), (61), (28), (10), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(54), (37), (35), (21), (14), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Functional Studies (Func), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Western Blotting (WB)
(290), (112), (91), (49), (46), (45), (39), (28), (23), (15), (10), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 9 references available
Quantity 0.1 mg
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Catalog No. ABIN638430
290.40 $
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Immunogen A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Clone MEM-18
Isotype IgG1
Specificity The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64. HLDA III, WS Code M 253HLDA IV, WS Code M 314HLDA V, WS Code M MA087HLDA VI, WS Code M MA95
Purification Purified by protein A
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 µm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Alternative Name CD14
Background CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor, it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Gene ID 929
Research Area Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation
Application Notes Flow Cytometry: Recommended dilution: 4 µg/mL
Western Blotting:
Application note: Non-reducing conditions.
ELISA: The antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody B-A8 -, labeled bacterial LPS to the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2 domain.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Product cited in: Bazil, Baudys, Hilgert et al.: "Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14." in: Molecular immunology, Vol. 26, Issue 7, pp. 657-62, 1989 (PubMed).

Bazil, Horejsí, Baudys et al.: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." in: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, 1987 (PubMed).

Funda, Tucková, Farré et al.: "CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited." in: Infection and immunity, Vol. 69, Issue 6, pp. 3772-81, 2001 (PubMed).

Stöckl, Majdic, Fischer et al.: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, 2001 (PubMed).

Sing, Rost, Tvardovskaia et al.: "Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression." in: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1017-24, 2002 (PubMed).

Iwaki, Nishitani, Mitsuzawa et al.: "The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain." in: Biochemical and biophysical research communications, Vol. 328, Issue 1, pp. 173-6, 2005 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Angel, Lala, Chen et al.: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, 2007 (PubMed).

Weiss, Lichtenauer, Kirchner et al.: "Hepatic progenitor cells from adult human livers for cell transplantation." in: Gut, Vol. 57, Issue 8, pp. 1129-38, 2008 (PubMed).

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