CD14 Molecule (CD14) antibody

Antigen: CD14 Molecule (CD14)

Synonyms: CD14

Clonality: Monoclonal (MEM-18)

Host: Please enquire

Reactivity: Primate, Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), ELISA, Functional Studies (Func)

Catalog no.: ABIN638430

Additional Information

Alternative name: CD14

Gene ID: 929

Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

Endotoxin Level: low endotoxin

Isotype: IgG1

Clone: MEM-18

Specificity: The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages, weakly on granulocytes, also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64. HLDA III, WS Code M 253, HLDA IV, WS Code M 314, HLDA V, WS Code M MA087, HLDA VI, WS Code M MA95

Application Details

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 micrometer filter sterilized. Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.

Preservative (Components): Azide free

Storage: Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot, store at -20°C. Avoid freeze/thaw cycles.

Research Area: CD Antigens

Restrictions: For Research Use only

Publications

Product: Bazil, Baudys, Hilgert et al.: "Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14." in: Molecular immunology, Vol. 26, Issue 7, pp. 657-62, 1989 (PubMed).

Bazil, Horejsí, Baudys et al.: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." in: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, 1987 (PubMed).

Funda, Tucková, Farré et al.: "CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited." in: Infection and immunity, Vol. 69, Issue 6, pp. 3772-81, 2001 (PubMed).

Stöckl, Majdic, Fischer et al.: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, 2001 (PubMed).

Sing, Rost, Tvardovskaia et al.: "Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression." in: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1017-24, 2002 (PubMed).

Iwaki, Nishitani, Mitsuzawa et al.: "The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain." in: Biochemical and biophysical research communications, Vol. 328, Issue 1, pp. 173-6, 2005 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Angel, Lala, Chen et al.: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, 2007 (PubMed).

Weiss, Lichtenauer, Kirchner et al.: "Hepatic progenitor cells from adult human livers for cell transplantation." in: Gut, Vol. 57, Issue 8, pp. 1129-38, 2008 (PubMed).