E2F Transcription Factor 1 (E2F1) (pHis357) antibody

Details for Product No. ABIN650836
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Antigen
Synonyms
CG6376, DRTF1/E2F, DmE2F-1, Dmel\\CG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f1, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)0 ... show more
CG6376, DRTF1/E2F, DmE2F-1, Dmel\\CG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f1, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729, RBAP1, RBBP3, RBP3, mKIAA4009, xE2F, ATE2FB, E2F TRANSCRIPTION FACTOR-1 E2F1, E2F transcription factor 1, E2FB, E2FB TRANSCRIPTION FACTOR show less
Epitope
pHis357
(29), (25), (21), (17), (15), (11), (7), (7), (6), (6), (6), (5), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(258), (93), (58), (27), (27), (25), (12), (1), (1), (1), (1), (1)
Host
Rabbit
(208), (50), (3), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(15), (12), (11), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5)
Application
Dot Blot (DB)
(182), (87), (60), (50), (44), (39), (23), (21), (13), (5), (3), (2), (2), (1)
Pubmed 4 references available
Quantity 400 μL
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Catalog No. ABIN650836
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Immunogen This E2F1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding H357 of human E2F1.
Clone RB07916
Isotype Ig
Specificity This Phospho-E2F1-H357 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding H357 of human E2F1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name E2F1
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Synonyms: Retinoblastoma-associated protein 1, RBAP-1, PBR3, PRB-binding protein E2F-1, Retinoblastoma-binding protein 3, RBBP-3, Transcription factor E2F1, E2F-1,RBBP3, E2F1
Molecular Weight 46920 DA
Gene ID 1869
UniProt Q01094
Research Area Transcription Factors, Phospho-specific antibodies, Signaling
Application Notes DB = 1:500
Restrictions For Research Use only
Format Liquid
Concentration 0.26 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-E2F Transcription Factor 1 (E2F1) (pHis357) antibody Dot blot analysis of Phospho-hE2F1-H357 Pab (ABIN650836) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/mL.
Background publications Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Yang, Feng, Jiang et al.: "miR-449a and miR-449b are direct transcriptional targets of E2F1 and negatively regulate pRb-E2F1 activity through a feedback loop by targeting CDK6 and CDC25A." in: Genes & development, Vol. 23, Issue 20, pp. 2388-93, 2009 (PubMed).

Pulikkan, Dengler, Peramangalam et al.: "Cell-cycle regulator E2F1 and microRNA-223 comprise an autoregulatory negative feedback loop in acute myeloid leukemia." in: Blood, Vol. 115, Issue 9, pp. 1768-78, 2010 (PubMed).

Paik, Wang, Liu et al.: "Regulation of E2F1-induced apoptosis by the nucleolar protein RRP1B." in: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6348-63, 2010 (PubMed).

Validation Images
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