Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B) (C-Term), (AA 950-979) antibody

Details for Product No. ABIN654447
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Antigen
Epitope
C-Term, AA 950-979
(7), (6), (3), (2), (1), (1)
Reactivity
Human
(17), (5), (4), (1)
Host
Rabbit
(15), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(17), (9), (8), (7), (3), (2)
Pubmed 4 references available
Quantity 400 µL
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Catalog No. ABIN654447
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Immunogen This INPP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 950-979 AA from the C-terminal region of human INPP5B.
Clone RB27636
Isotype Ig
Specificity This INPP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 957-987 amino acids from the C-terminal region of human INPP5B.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name INPP5B
Background Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in response to extracellular signals. The IP signaling molecules are inactivated by a family of inositol polyphosphate-5-phosphatases (5-phosphatases). INPP5B encodes the type II 5-phosphatase. The protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq].
Synonyms: INPP5B,Type II inositol-1,4,5-trisphosphate 5-phosphatase,
Molecular Weight 112852 DA
Gene ID 3633
NCBI Accession NP_005531
UniProt P32019
Research Area Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B) (C-Term), (AA 950-979) antibody INPP5B Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the INPP5B antibody detected the INPP5B protein (arrow).
anti-Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B) (C-Term), (AA 950-979) antibody (2) INPP5B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the INPP5B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B) (C-Term), (AA 950-979) antibody (3) INPP5B Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Jänne, Rochelle, Martin-DeLeon et al.: "Mapping of the 75-kDa inositol polyphosphate-5-phosphatase (Inpp5b) to distal mouse chromosome 4 and its exclusion as a candidate gene for dysgenetic lens." in: Genomics, Vol. 28, Issue 2, pp. 280-5, 1996 (PubMed).

Williams, Choudhury, McKenzie et al.: "Targeting of the type II inositol polyphosphate 5-phosphatase INPP5B to the early secretory pathway." in: Journal of cell science, Vol. 120, Issue Pt 22, pp. 3941-51, 2007 (PubMed).

Mao, Balkin, Zoncu et al.: "A PH domain within OCRL bridges clathrin-mediated membrane trafficking to phosphoinositide metabolism." in: The EMBO journal, Vol. 28, Issue 13, pp. 1831-42, 2009 (PubMed).

Coon, Mukherjee, Hanna et al.: "Lowe syndrome patient fibroblasts display Ocrl1-specific cell migration defects that cannot be rescued by the homologous Inpp5b phosphatase." in: Human molecular genetics, Vol. 18, Issue 23, pp. 4478-91, 2009 (PubMed).

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