INPP5B antibody (Middle Region)

Catalog No. ABIN2785875

Cited in 1 publication. The Rabbit Polyclonal anti-INPP5B antibody (ABIN2785875) specifically detects INPP5B in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Guinea Pig and Horse samples.

Quick Overview for INPP5B antibody (Middle Region) (ABIN2785875)

Target: INPP5B (Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INPP5B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-INPP5B Antibody

Binding Specificity: Middle Region

Purpose: INPP5B Antibody - middle region

Sequence: IHNGQVPCHF EFINKPDEES YCKQWLNANP SRGFLLPDSD VEIDLELFVN

Predicted Reactivity: Dog: 85%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 92%, Rabbit: 77%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against INPP5B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human INPP5B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54772-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-INPP5B antibody (ABIN2785875)

Tothova, Kollipara, Huntly, Lee, Castrillon, Cullen, McDowell, Lazo-Kallanian, Williams, Sears, Armstrong, Passegué, DePinho, Gilliland: "FoxOs are critical mediators of hematopoietic stem cell resistance to physiologic oxidative stress." in: Cell, Vol. 128, Issue 2, pp. 325-39, (2007) (PubMed).

Target Details for INPP5B

Target: INPP5B (Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B))

Alternative Name: INPP5B

Background:

Background Information: Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in response to extracellular signals. The IP signaling molecules are inactivated by a family of inositol polyphosphate-5-phosphatases (5-phosphatases). INPP5B is the type II 5-phosphatase. The protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus.Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in response to extracellular signals. The IP signaling molecules are inactivated by a family of inositol polyphosphate-5-phosphatases (5-phosphatases). This gene encodes the type II 5-phosphatase. The protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Gene Name: Inositol polyphosphate-5-phosphatase, 75 kDa

Alternative Symbols: 5PTase

Protein Name: Type II inositol 1,4,5-trisphosphate 5-phosphatase

Molecular Weight: 77kDa

Gene ID: 3633

NCBI Accession: NP_005531

UniProt: P32019