Lysyl-tRNA Synthetase (KARS) (N-Term), (AA 70-98) antibody

Details for Product No. ABIN655049
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Antigen
Epitope
N-Term, AA 70-98
(6), (6), (2), (2), (1), (1)
Reactivity
Human
(38), (18), (14), (13), (12), (12), (12)
Host
Rabbit
(30), (10)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(30), (13), (12), (12), (10), (4), (4), (3), (2), (1), (1)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN655049
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Immunogen This KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 AA from the N-terminal region of human KARS.
Clone RB14918
Isotype Ig
Specificity This KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human KARS.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name KARS
Background Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Synonyms: Lysyl-tRNA synthetase,KARS,KIAA0070
Molecular Weight 68048 DA
Gene ID 3735
NCBI Accession NP_005539, NP_001123561
UniProt Q15046
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Chromatin
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Lysyl-tRNA Synthetase (KARS) (N-Term), (AA 70-98) antibody KARS Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the KARS antibody detected the KARS protein (arrow).
anti-Lysyl-tRNA Synthetase (KARS) (N-Term), (AA 70-98) antibody (2) Western blot analysis of KARS (arrow) using rabbit polyclonal KARS Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KARS gene.
anti-Lysyl-tRNA Synthetase (KARS) (N-Term), (AA 70-98) antibody (3) KARS Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Kawamata, Magrané, Kunst et al.: "Lysyl-tRNA synthetase is a target for mutant SOD1 toxicity in mitochondria." in: The Journal of biological chemistry, Vol. 283, Issue 42, pp. 28321-8, 2008 (PubMed).

McLaughlin, Sakaguchi, Liu et al.: "Compound heterozygosity for loss-of-function lysyl-tRNA synthetase mutations in a patient with peripheral neuropathy." in: American journal of human genetics, Vol. 87, Issue 4, pp. 560-6, 2010 (PubMed).

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