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Lysyl-tRNA Synthetase (KARS) (N-Term), (AA 70-98) antibody

Details for Product No. ABIN655049
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Antigen
Epitope
N-Term, AA 70-98
(5), (4), (2), (2)
Reactivity
Human
(34), (18), (14), (13), (12), (12), (12)
Host
Rabbit
(28), (9)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(27), (15), (10), (10), (9), (3), (2), (2), (1)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN655049
291.50 $
Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 AA from the N-terminal region of human KARS.
Clone RB14918
Isotype Ig
Specificity This KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human KARS.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name KARS
Background Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Synonyms: Lysyl-tRNA synthetase,KARS,KIAA0070
Molecular Weight 68048 DA
Gene ID 3735
UniProt Q15046, NP_005539.1, NP_001123561.1
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Chromatin
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Lysyl-tRNA Synthetase (KARS) (N-Term), (AA 70-98) antibody KARS Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the KARS antibody detected the KARS protein (arrow).
anti-Lysyl-tRNA Synthetase (KARS) (N-Term), (AA 70-98) antibody (2) Western blot analysis of KARS (arrow) using rabbit polyclonal KARS Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KARS gene.
anti-Lysyl-tRNA Synthetase (KARS) (N-Term), (AA 70-98) antibody (3) KARS Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Kawamata, Magrané, Kunst et al.: "Lysyl-tRNA synthetase is a target for mutant SOD1 toxicity in mitochondria." in: The Journal of biological chemistry, Vol. 283, Issue 42, pp. 28321-8, 2008 (PubMed).

McLaughlin, Sakaguchi, Liu et al.: "Compound heterozygosity for loss-of-function lysyl-tRNA synthetase mutations in a patient with peripheral neuropathy." in: American journal of human genetics, Vol. 87, Issue 4, pp. 560-6, 2010 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN655049
291.50 $
Plus shipping costs $45.00
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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