KARS antibody (C-Term)

Catalog No. ABIN2778941

The Rabbit Polyclonal anti-KARS antibody is suitable to detect KARS in samples from Human, Mouse, Rat, Guinea Pig, Horse, Pig, Rabbit and Cow. It has been validated for WB and IHC.

Quick Overview for KARS antibody (C-Term) (ABIN2778941)

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Pig, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KARS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-KARS Antibody

Binding Specificity: C-Term

Purpose: KARS Antibody - C-terminal region

Sequence: GMGIDRVAMF LTDSNNIKEV LLFPAMKPED KKENVATTDT LESTTVGTSV

Predicted Reactivity: Cow: 92%, Guinea Pig: 85%, Horse: 88%, Human: 100%, Mouse: 79%, Pig: 86%, Rabbit: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against KARS. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human KARS

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8104721

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for KARS

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Alternative Name: KARS

Background:

Background Information: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

Gene Name: Lysyl-tRNA synthetase

Alternative Symbols: KRS, KARS, KARS2, LEPID, CMTRIB, DEAPLE, DFNB89

Protein Name: Lysine--tRNA ligase

Molecular Weight: 66kDa

Gene ID: 3735

NCBI Accession: NP_005539

UniProt: Q15046