Nibrin (NBN) (C-Term), (AA 602-630) antibody

Details for Product No. ABIN655721
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Antigen
Synonyms AT-V1, AT-V2, ATV, NBS, NBS1, P95, Nbs1, NBN
Epitope
C-Term, AA 602-630
(48), (16), (8), (7), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(205), (78), (54), (24), (24), (24)
Host
Rabbit
(166), (42)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
(167), (70), (43), (39), (30), (24), (20), (17), (5), (2), (2), (1)
Pubmed 3 references available
Catalog no. ABIN655721
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This NBN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 602-630 AA from the C-terminal region of human NBN.
Clone RB19072
Isotype Ig
Specificity This NBN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 609-638 amino acids from the C-terminal region of human NBN.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NBN
Background Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
Synonyms: NBN,Nibrin,NBS, NBS1, P95
Molecular Weight 84959 DA
Gene ID 4683
UniProt O60934, NP_002476.2
Research Area Phospho-specific antibodies, Protein Modifications, Cell Cycle, Chromatin, Signaling, Cell Structure
Application Notes WB = 1:1000, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.27 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Nibrin (NBN) (C-Term), (AA 602-630) antibody NBN Antibody (C-term) western blot analysis in NCI-H460,NCI-H292 cell line lysates (35ug/lane).This demonstrates the NBN antibody detected the NBN protein (arrow).
anti-Nibrin (NBN) (C-Term), (AA 602-630) antibody (2) Confocal immunofluorescent analysis of NBN Antibody (C-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
anti-Nibrin (NBN) (C-Term), (AA 602-630) antibody (3) NBN Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Liu, Shete, Wang et al.: "Gamma-radiation sensitivity and polymorphisms in RAD51L1 modulate glioma risk." in: Carcinogenesis, Vol. 31, Issue 10, pp. 1762-9, 2010 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).

Kavitha, Choudhary, Raghavan et al.: "Differential regulation of MRN (Mre11-Rad50-Nbs1) complex subunits and telomerase activity in cancer cells." in: Biochemical and biophysical research communications, Vol. 399, Issue 4, pp. 575-80, 2010 (PubMed).

Hosts (166), (42)
Reactivities (205), (78), (54), (24), (24), (24)
Applications (167), (70), (43), (39), (30), (24), (20), (17), (5), (2), (2), (1)
Conjugates (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Epitopes (48), (16), (8), (7), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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