Mediator Complex Subunit 8 (MED8) (AA 95-122), (Center) antibody

Details for Product No. ABIN656578
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Antigen
Epitope
AA 95-122, Center
(8), (8), (3), (3), (1), (1), (1)
Reactivity
Human
(21), (6), (5), (1), (1)
Host
Rabbit
(15), (6)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(21), (15), (1), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN656578
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Immunogen This MED8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-122 AA from the Central region of human MED8.
Clone RB20608
Isotype Ig
Specificity This MED8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the Central region of human MED8.
Predicted Reactivity Mouse (Murine),Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name MED8
Background This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.
Synonyms: Mediator of RNA polymerase II transcription subunit 8,MED8,
Molecular Weight 29080 DA
Gene ID 112950
NCBI Accession NP_443109, NP_963836
UniProt Q96G25
Research Area Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.48 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment MED8 Antibody (Center) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
anti-Mediator Complex Subunit 8 (MED8) (AA 95-122), (Center) antibody MED8 Antibody (Center) (ABIN656578) western blot analysis in HL-60 cell line lysates (35 µg/lane). This demonstrates the MED8 antibody detected the MED8 protein (arrow).
Background publications Sato, Tomomori-Sato, Banks et al.: "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex." in: The Journal of biological chemistry, Vol. 278, Issue 50, pp. 49671-4, 2003 (PubMed).

Tomomori-Sato, Sato, Parmely et al.: "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5846-51, 2004 (PubMed).

Sato, Tomomori-Sato, Parmely et al.: "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." in: Molecular cell, Vol. 14, Issue 5, pp. 685-91, 2004 (PubMed).

Wang, Yoon, Oh et al.: "Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags." in: Biochemical and biophysical research communications, Vol. 345, Issue 3, pp. 1022-32, 2006 (PubMed).

Léonard, Kohl, Hart et al.: "Interaction of Bunyamwera Orthobunyavirus NSs protein with mediator protein MED8: a mechanism for inhibiting the interferon response." in: Journal of virology, Vol. 80, Issue 19, pp. 9667-75, 2006 (PubMed).

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