MED8 antibody (AA 1-195)

Catalog No. ABIN5519031

This Rabbit Polyclonal antibody specifically detects MED8 in WB, ELISA, FACS, IF, ICC and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MED8 antibody (AA 1-195) (ABIN5519031)

Target: MED8 (Mediator Complex Subunit 8 (MED8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Product Details anti-MED8 Antibody

Binding Specificity: AA 1-195

Purpose: Anti-MED8 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MED8 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MED8 recombinant protein (Position: M1-R195).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MED8

Target: MED8 (Mediator Complex Subunit 8 (MED8))

Alternative Name: MED8

Background:

Background: Mediator of RNA polymerase II transcription subunit 8 is an enzyme that in humans is encoded by the MED8 gene. This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation.

Gene Full Name: mediator complex subunit 8

Molecular Weight: 29 kDa

Gene ID: 112950

UniProt: Q96G25

Pathways: Regulation of Lipid Metabolism by PPARalpha