MED8 antibody

Catalog No. ABIN7074582

The Rabbit Polyclonal anti-MED8 antibody is suitable to detect MED8 in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC.

Quick Overview for MED8 antibody (ABIN7074582)

Target: MED8 (Mediator Complex Subunit 8 (MED8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-MED8 Antibody

Cross-Reactivity: Human, Rat

Purification: Affinity purification

Immunogen: Recombinant protein corresponding to Mouse MED8

Application Details

Application Notes: WB (H,M,R) 1:500-1:1000, IHC/IF (M,R) 1:600-1:1200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for MED8

Target: MED8 (Mediator Complex Subunit 8 (MED8))

Alternative Name: MED8

Background: MED8, a component of the Mediator complex, is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It plays a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. MED8 has three isoforms with MW 19 kDa, 29 kDa and 33 kDa. And the ubiquitination form will be 38-43 kDa.

Molecular Weight: 29 kDa

Gene ID: 80509

NCBI Accession: NP_001277617

UniProt: Q9D7W5

Pathways: Regulation of Lipid Metabolism by PPARalpha