Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2) (C-Term), (AA 312-340) antibody

Details for Product No. ABIN657484
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Antigen
Synonyms NF-E2, p45, 45kDa, p45NFE2, p45nf-e2
Epitope
C-Term, AA 312-340
(7), (7), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(19), (4), (3), (1), (1)
Host
Rabbit
(17), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(20), (11), (4), (3), (1), (1), (1)
Pubmed 5 references available
Quantity 400 μL
Options
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Catalog No. ABIN657484
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Immunogen This NFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-340 AA from the C-terminal region of human NFE2.
Clone RB31295
Isotype Ig
Specificity This NFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-340 amino acids from the C-terminal region of human NFE2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NFE2
Background Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
Synonyms: Transcription factor NF-E2 45 kDa subunit,NFE2,
Molecular Weight 41473 DA
Gene ID 4778
NCBI Accession NP_006154, NP_001129495
UniProt Q16621
Research Area Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment NFE2 Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
anti-Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2) (C-Term), (AA 312-340) antibody NFE2 Antibody (C-term) (ABIN657484) western blot analysis in MDA-MB435 cell line lysates (35 µg/lane). This demonstrates the NFE2 antibody detected the NFE2 protein (arrow).
Background publications Soranzo, Spector, Mangino et al.: "A genome-wide meta-analysis identifies 22 loci associated with eight hematological parameters in the HaemGen consortium." in: Nature genetics, Vol. 41, Issue 11, pp. 1182-90, 2009 (PubMed).

Motohashi, Kimura, Fujita et al.: "NF-E2 domination over Nrf2 promotes ROS accumulation and megakaryocytic maturation." in: Blood, Vol. 115, Issue 3, pp. 677-86, 2010 (PubMed).

Lee, Shyu, Hsu et al.: "JNK-mediated turnover and stabilization of the transcription factor p45/NF-E2 during differentiation of murine erythroleukemia cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 1, pp. 52-7, 2010 (PubMed).

Zhou, Li, Deng et al.: "USF and NF-E2 cooperate to regulate the recruitment and activity of RNA polymerase II in the beta-globin gene locus." in: The Journal of biological chemistry, Vol. 285, Issue 21, pp. 15894-905, 2010 (PubMed).

Wang, Schwemmers, Hexner et al.: "AML1 is overexpressed in patients with myeloproliferative neoplasms and mediates JAK2V617F-independent overexpression of NF-E2." in: Blood, Vol. 116, Issue 2, pp. 254-66, 2010 (PubMed).

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