NFE2 antibody (Center)

Catalog No. ABIN2855460

Product Details anti-NFE2 Antibody

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFE2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Cross-Reactivity: Monkey, Pig (Porcine)

Cross-Reactivity (Details): Monkey (97 %), Pig (91 %)

Characteristics: Rabbit Polyclonal antibody to NF-E2 (nuclear factor (erythroid-derived 2), 45 kDa)
NF-E2 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NF-E2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation Assay-dependent dilution Western blot 1:500-1:3000* ChIP-sequencing Assay-dependent dilution Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* ImmunoprecipitationAssay-dependent dilution Western blot1:500-1:3000* ChIP-sequencingAssay-dependent dilution

Comment:

Positive Control: 293T , mouse spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.03 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NFE2

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Alternative Name: NF-E2 (NFE2 Products)

Synonyms: NF-E2 antibody, p45 antibody, 45kDa antibody, p45NFE2 antibody, p45nf-e2 antibody, nuclear factor, erythroid 2 antibody, nuclear factor, erythroid derived 2 antibody, NFE2 antibody, Nfe2 antibody

Background: Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

Cellular Localization: Nucleus

Molecular Weight: 41 kDa

Gene ID: 4778