MTOR Associated Protein, LST8 Homolog (S. Cerevisiae) (MLST8) antibody

Details for Product No. ABIN659124
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Synonyms Gbl, AA409454, AI505104, AI851821, 0610033N12Rik, GBL, GbetaL, LST8, POP3, WAT1, gbl, lts8
Mouse (Murine)
(54), (31), (27), (14), (13), (11), (3), (2), (1), (1), (1)
(52), (5), (1)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(46), (14), (12), (12), (9), (6), (4), (1)
Pubmed 2 references available
Catalog no. ABIN659124
Quantity 0.1 mL
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Immunogen This GBL monoclonal antibody is generated from mouse immunized with GBL recombinant protein.
Clone 211CT7-1-1
Isotype IgG1, kappa
Specificity This GBL monoclonal antibody is generated from mouse immunized with GBL recombinant protein.
Purification Ascites
Alternative Name GBL
Background Subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with FRAP1 and enhances its kinase activity. In nutrient-poor conditions, stabilizes the FRAP1-RPTOR interaction and favors RPTOR-mediated inhibition of FRAP1 activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
Synonyms: Target of rapamycin complex subunit LST8,MLST8,GBL, LST8
Molecular Weight 35876 DA
Gene ID 64223
UniProt Q9BVC4, NP_071767.3
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes WB = 1:100-8000
Restrictions For Research Use only
Format Liquid
Buffer Crude ascites with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment GBL Antibody (ascites) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
anti-MTOR Associated Protein, LST8 Homolog (S. Cerevisiae) (MLST8) antibody GBL western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the GBL antibody detected the GBL protein (arrow).
Background publications Jacinto, Loewith, Schmidt et al.: "Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive." in: Nature cell biology, Vol. 6, Issue 11, pp. 1122-8, 2004 (PubMed).

Ali, Sabatini: "Structure of S6 kinase 1 determines whether raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site." in: The Journal of biological chemistry, Vol. 280, Issue 20, pp. 19445-8, 2005 (PubMed).

Hosts (52), (5), (1)
Reactivities (54), (31), (27), (14), (13), (11), (3), (2), (1), (1), (1)
Applications (46), (14), (12), (12), (9), (6), (4), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (11), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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