MTOR Associated Protein, LST8 Homolog (S. Cerevisiae) (MLST8) antibody

Antigen: MTOR Associated Protein, LST8 Homolog (S. Cerevisiae) (MLST8)

Clonality: Monoclonal

Host: Mouse

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659124

Additional Information

Alternative name: GBL

Gene ID: 64223

UniProt: Q9BVC4, NP_071767.3

Immunogen: This GBL monoclonal antibody is generated from mouse immunized with GBL recombinant protein.

Isotype: IgG1 kappa

Description: Other names: Target of rapamycin complex subunit LST8,MLST8,GBL, LST8

Characteristics: Mouse Monoclonal Antibody (Mab)

Specificity: This GBL monoclonal antibody is generated from mouse immunized with GBL recombinant protein.

Molecular Weight: 35876 DA

Comments:

Background: Subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with FRAP1 and enhances its kinase activity. In nutrient-poor conditions, stabilizes the FRAP1-RPTOR interaction and favors RPTOR-mediated inhibition of FRAP1 activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1000 Western blotting~~1:100~8000

Concentration: Crude ascites

Buffer: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Storage: GBL Antibody (ascites) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Jacinto, Loewith, Schmidt et al.: "Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive." in: Nature cell biology, Vol. 6, Issue 11, pp. 1122-8, 2004 (PubMed).

Ali, Sabatini: "Structure of S6 kinase 1 determines whether raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site." in: The Journal of biological chemistry, Vol. 280, Issue 20, pp. 19445-8, 2005 (PubMed).