CD25 antibody (APC)
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- Target See all CD25 (IL2RA) Antibodies
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD25 antibody is conjugated to APC
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Application
- Flow Cytometry (FACS)
- Specificity
- Human IL2RA / CD25
- Purification
- Protein A/G purified
- Sterility
- Sterile filtered
- Immunogen
- PHA-activated human T-lymphocytes.
- Clone
- ITYV
- Isotype
- IgG1
- Top Product
- Discover our top product IL2RA Primary Antibody
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- Application Notes
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Approved: Flo
Usage: PBMC: Add 10 μL of antibody/10^6 PBMC in 100 μL PBS. Mix gently and incubate for 15 minutes at 2 to 8 °C. Wash twice with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 μL of antibody/100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 °C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.08 % sodium azide, 0.2 % carrier protein, sterile-filtered
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 4°C. Do not freeze.
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- Target
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Alternative Name
- IL2RA / CD25 (IL2RA Products)
- Background
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Name/Gene ID: IL2RA
Family: Interleukin
Synonyms: IL2RA, CD25 antigen, CD25, IDDM10, IL-2 receptor subunit alpha, IL2R, Interleukin 2 receptor, alpha, IL-2-RA, IL2-RA, TCGFR, IL-2R subunit alpha, p55, TAC antigen - Gene ID
- 3559
- UniProt
- P01589
- Pathways
- JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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