CD105 (Endoglin / TGF-Beta1/3 Receptor) antibody (Biotin)

Details for Product No. ABIN810077
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Antigen
Synonyms CD105, AI528660, AI662476, S-endoglin, END, HHT1, ORW1
Reactivity
Human, Pig (Porcine)
(224), (94), (35), (33), (25), (13), (13), (6), (1)
Host
Mouse
(142), (118), (40), (2), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
(31), (26), (18), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
(187), (162), (64), (57), (46), (42), (37), (29), (21), (15), (7), (7), (5), (4), (4), (1), (1)
Pubmed 5 references available
Catalog no. ABIN810077
Quantity 0.1 mg
Price
363.00 $   Plus shipping costs $45.00
Options
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.
Clone MEM-229
Isotype IgG2a
Specificity The antibody MEM-229 recognizes CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow, it is also present on syncytiotrophoblast on placenta throughout pregnancy.
Characteristics The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Purification Purified from ascites by protein-A affinity chromatography.
Alternative Name CD105
Background CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
Gene ID 2022
Research Area Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Product cited in: Li, Issa, Kumar et al.: "CD105 prevents apoptosis in hypoxic endothelial cells." in: Journal of cell science, Vol. 116, Issue Pt 13, pp. 2677-85, 2003 (PubMed).

Zhu, Sun, Xie et al.: "Hypoxic induction of endoglin via mitogen-activated protein kinases in mouse brain microvascular endothelial cells." in: Stroke; a journal of cerebral circulation, Vol. 34, Issue 10, pp. 2483-8, 2003 (PubMed).

Guo, Slevin, Li et al.: "CD105 inhibits transforming growth factor-beta-Smad3 signalling." in: Anticancer research, Vol. 24, Issue 3a, pp. 1337-45, 2004 (PubMed).

Warrington, Hillarby, Li et al.: "Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells." in: Anticancer research, Vol. 25, Issue 3B, pp. 1851-64, 2005 (PubMed).

Piao, Tokunaga: "Significant expression of endoglin (CD105), TGFbeta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study." in: Journal of atherosclerosis and thrombosis, Vol. 13, Issue 2, pp. 82-9, 2006 (PubMed).

Hosts (142), (118), (40), (2), (2)
Reactivities (224), (94), (35), (33), (25), (13), (13), (6), (1)
Applications (187), (162), (64), (57), (46), (42), (37), (29), (21), (15), (7), (7), (5), (4), (4), (1), (1)
Conjugates (31), (26), (18), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Epitopes (9), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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