Endoglin (ENG) antibody

Details for Product No. ABIN93939
Antigen
Reactivity
Human, Rat (Rattus)
455
174
82
19
14
8
5
5
5
4
1
Host
Mouse
260
249
47
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
56
52
46
22
8
8
7
7
7
7
7
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Microcytotoxicity Assay (MCA), Western Blotting (WB)
352
340
130
115
79
72
47
38
26
21
17
13
12
7
7
6
4
3
3
2
2
2
2
1
Options
Immunogen Recombinant Vaccinia virus containing the human CD105 cDNA.
Clone MEM-226
Isotype IgG2a
Specificity The antibody MEM-226 reacts with CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow, it is also present on syncytiotrophoblast on placenta throughout pregnancy.
Cross-Reactivity (Details) Human, Rat
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD105 (ENG Antibody Abstract)
Background CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Endoglin (ENG) antibody (ABIN93939) Surface staining (flow cytometry) of HUVEC (human umbilical vein endothelial cells) w...
Image no. 2 for anti-Endoglin (ENG) antibody (ABIN93939) Surface staining (mass cytometry) of hTERT cell line with anti-human CD105 () 143Nd. ...
Product cited in: Schmidt, Achermann, Odermatt, Breymann, Mol, Genoni, Zund, Hoerstrup: "Prenatally fabricated autologous human living heart valves based on amniotic fluid derived progenitor cells as single cell source." in: Circulation, Vol. 116, Issue 11 Suppl, pp. I64-70, 2007 (PubMed).