RBCL (Isoform 1) antibody

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Isoform 1
Arabidopsis thaliana, Lobaria pulmonaria, Pea (Pisum sativum), Potato (Solanum tuberosum), Spinach (Spinacia oleracea)
Immunolocalization (IL), Western Blotting (WB)
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Immunogen KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana UniProt: O03042, TAIR: AtCg00490, Synechococcus sp. Q3ALL1
Characteristics Expected / apparent Molecular Weight of the Antigene: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
Purification affinity purified
Background AGI Code: ATCG00490
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.
Molecular Weight 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
UniProt Q3ALL1, O03042
Application Notes Recommended Dilution: 1 : 10 000 - 1 : 20 000 on 2 µg of total cellular protein, detected with standard ECL(WB), (IL) tested on a grass species, formaldehyde-fixed and paraffin-embeddedtissue following the protocol from Gonzalez et al. (1998) Plant Physiol. V. 116.

This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.

Restrictions For Research Use only
Format Liquid
Reconstitution For reconstitution add 225 µL of milliQ water per each tube
Concentration 16 µg/µL
Buffer PBS pH 8.0+ 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Make aliquots to avoid working with a stock.
Please, Remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubek.
Storage 4 °C
Product cited in: Abdel-Ghany, Pilon: "MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15932-45, 2008 (PubMed).

Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, 2008 (PubMed).

Menon, Dou, Heinhorst, Shively, Cannon: "Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species." in: PLoS ONE, Vol. 3, Issue 10, pp. e3570, 2008 (PubMed).

Background publications Soares, Driscoll, Olmos, Harbinson, Arrabaca, Foyer: "Adaxial/abaxial specification in the regulation of photosynthesis and stomatal opening with respect to light orientation and growth with CO2 enrichment in the C4 species Paspalum dilatatum." in: The New phytologist, Vol. 177, Issue 1, pp. 186-98, 2007 (PubMed).

Mackenzie, Johnson, Campbell: "Dynamics of fluxes through photosynthetic complexes in response to changing light and inorganic carbon acclimation in Synechococcus elongatus." in: Photosynthesis research, Vol. 85, Issue 3, pp. 341-57, 2005 (PubMed).