RBCL (Isoform 1), (Isoform 2) antibody

Details for Product No. ABIN93539
Antigen
Epitope
Isoform 1, Isoform 2
4
1
1
Reactivity
Arabidopsis thaliana, Baculogypsina sphaerulata
4
1
1
1
1
1
1
Host
Rabbit
5
1
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
Application
Confocal Microscopy (CM), Immunofluorescence (IF), Labeling (Lbl), Tissue Printing (TP), Western Blotting (WB)
4
3
1
Options
Immunogen KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), including Arabidopsis thaliana AtCg00490, Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhardtii P00877, Synechococcus PCC 7920 A5CKC5
Specificity Antibody is a cellular [compartment marker] of plastid stroma or a cytopasmmarker in cyanobacteria. Antibody can detect Rubisco protein from 31.25 fmoles. Antibody will detect Rubisco Form I and Form II, therefore is suitable for workwith samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms,Raphidophytes, brown algae) as well as higher plants. This antibody can be usedto quantify Rubisco protein in plant and algal samples.
Characteristics Expected / apparent Molecular Weight of the Antigene: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
Purification serum
Antigen
Background AGI Code: ATCG00490
This antibody is especially suitable for quantifying of Rubisco in plant and algal samples. Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.
Molecular Weight 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
UniProt P00877, P05698, O03042, P0C510
Application Notes Recommended Dilution: 1 : 5000 - 10 000 with standard ECL (WB), 1 : 800 (TP),immunofluorescence,confocal microscopy (IF), 1 : 250 for images see Prins et al.(2008), detailed protocol available on request (IL).
Comment

This antibody was used in:Immunocytochemical staining of diatoms according to Schmid (2003) J Phycol 39: 139-153 and Wordemann et al. (1986) J Cell Biol 102: 1688-1698.Immunofluorescence Dreier et al. (2012). FEMS Microbial Ecol., March 2012.Western blot and tissue printing during a student course Ma et al. (2009).

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 10 µL of sterile water
Buffer PBS pH 7.4
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage -20 °C
Product cited in: Seveso, Montano, Strona, Orlandi, Galli, Vai: "The susceptibility of corals to thermal stress by analyzing Hsp60 expression." in: Marine environmental research, Vol. 99C, pp. 69-75, 2014 (PubMed).

Scotti, Sannino, Idoine, Hamman, De Stradis, Giorio, Maréchal-Drouard, Bock, Cardi: "The HIV-1 Pr55(gag) polyprotein binds to plastidial membranes and leads to severe impairment of chloroplast biogenesis and seedling lethality in transplastomic tobacco plants." in: Transgenic research, 2014 (PubMed).

Kolesinski, Belusiak, Czarnocki-Cieciura, Szczepaniak: "Rubisco Accumulation Factor 1 from Thermosynechococcus elongatus participates in the final stages of ribulose-1,5-bisphosphate carboxylase/oxygenase assembly in Escherichia coli cells and in vitro." in: The FEBS journal, Vol. 281, Issue 17, pp. 3920-32, 2014 (PubMed).

Niewiadomska, Bilger, Gruca, Mulisch, Miszalski, Krupinska: "CAM-related changes in chloroplastic metabolism of Mesembryanthemum crystallinum L." in: Planta, Vol. 233, Issue 2, pp. 275-85, 2011 (PubMed).

Balsemão-Pires, Jaillais, Olson, Andrade, Umen, Chory, Sachetto-Martins: "The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism." in: BMC plant biology, Vol. 11, pp. 108, 2011 (PubMed).

Keech, Pesquet, Gutierrez, Ahad, Bellini, Smith, Gardeström: "Leaf senescence is accompanied by an early disruption of the microtubule network in Arabidopsis." in: Plant physiology, Vol. 154, Issue 4, pp. 1710-20, 2010 (PubMed). Method employed by authors: Western Blotting (WB) (1:5000).

Masuda, Mizusawa, Narisawa, Tozawa, Ohta, Takamiya: "The bacterial stringent response, conserved in chloroplasts, controls plant fertilization." in: Plant & cell physiology, Vol. 49, Issue 2, pp. 135-41, 2008 (PubMed).

Prins, van Heerden, Olmos, Kunert, Foyer: "Cysteine proteinases regulate chloroplast protein content and composition in tobacco leaves: a model for dynamic interactions with ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) vesicular bodies." in: Journal of experimental botany, Vol. 59, Issue 7, pp. 1935-50, 2008 (PubMed).

Wordeman, McDonald, Cande: "The distribution of cytoplasmic microtubules throughout the cell cycle of the centric diatom Stephanopyxis turris: their role in nuclear migration and positioning the mitotic spindle during cytokinesis." in: The Journal of cell biology, Vol. 102, Issue 5, pp. 1688-98, 1986 (PubMed).