alpha Tubulin (TUBA1) (N-Term) antibody

Details for Product No. ABIN93891
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Antigen
Synonyms K-ALPHA-1, TUBA2, mec-12, tuba4
Epitope
N-Term
(12), (8), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
General
(84), (36), (33), (23), (17), (13), (7), (6), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(70), (28), (12), (9), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(105), (36), (34), (30), (29), (25), (20), (10), (6), (6), (2), (2), (1), (1)
Pubmed 17 references available
Quantity 0.1 mg
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Catalog No. ABIN93891
333.96 $
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Immunogen Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
Clone TU-01
Isotype IgG1
Specificity The antibody TU-01 recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
Purification Purified from ascites by precipitation methods.
Purity > 95 % (by SDS-PAGE)
Alternative Name alpha-tubulin
Background The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening -, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression.Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.
Application Notes Western Blotting: Recommended dilution: 1-2 µg/mL,
Incubation 60 min in room temperature
Positive control: HPB-ALL human peripheral blood leukemia cell line (
Incubation 60 min) Porcine brain lysate (
Incubation 90 min)
Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Application note: Reducing conditions.
Immunohistochemistry (paraffin sections) Recommended dilution: 5 µg/mL
Positive tissue: heart.
Immunocytochemistry
Staining technique: fixed and permeabilized cells

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
anti-alpha Tubulin (TUBA1) (N-Term) antibody Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01, green) and anti-Vimentin (VI-01, cat. no. 11-254-C100, red). Nucleus is stained with DAPI (blue).
anti-alpha Tubulin (TUBA1) (N-Term) antibody (2) Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (TU-01, red). Nucleus is stained with DAPI (blue).
Product cited in: Cancer: Krupkova, Loja, Redova et al.: "Analysis of nuclear nestin localization in cell lines derived from neurogenic tumors." in: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2011 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Hrdinka, Dráber, Stepánek et al.: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011 (PubMed).

Sana, Zambo, Skoda et al.: "CD133 expression and identification of CD133/nestin positive cells in rhabdomyosarcomas and rhabdomyosarcoma cell lines." in: Analytical cellular pathology (Amsterdam), 2011 (PubMed).

Lueck, Hennig, Lommatzsch et al.: "Complement and UV-Irradiated Photoreceptor Outer Segments Increase the Cytokine Secretion by Retinal Pigment Epithelial Cells." in: Investigative ophthalmology & visual science, Vol. 53, Issue 3, pp. 1406-13, 2012 (PubMed).

Dolezalova, Mraz, Barta et al.: "MicroRNAs regulate p21(Waf1/Cip1) protein expression and the DNA damage response in human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 30, Issue 7, pp. 1362-72, 2012 (PubMed).

General Dráber, Dráberová, Viklický: "Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head." in: Histochemistry, Vol. 95, Issue 5, pp. 519-24, 1991 (PubMed).

Dráber, Dráberová, Linhartová et al.: "Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies." in: Journal of cell science, Vol. 92 ( Pt 3), pp. 519-28, 1990 (PubMed).

Dráber, Dráberová, Zicconi et al.: "Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin." in: European journal of cell biology, Vol. 41, Issue 1, pp. 82-8, 1987 (PubMed).

Smertenko, Blume, Viklický et al.: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." in: Planta, Vol. 201, Issue 3, pp. 349-58, 1997 (PubMed).

Smertenko, Blume, Viklický et al.: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, 1997 (PubMed).

Tsao, Tsai, Tung et al.: "Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon." in: Molecular and cellular biochemistry, Vol. 327, Issue 1-2, pp. 163-70, 2009 (PubMed).

Miyajima, Maruyama, Nonomura et al.: "TRIM36 interacts with the kinetochore protein CENP-H and delays cell cycle progression." in: Biochemical and biophysical research communications, Vol. 381, Issue 3, pp. 383-7, 2009 (PubMed).

Lukas, Mazna, Valenta et al.: "Dazap2 modulates transcription driven by the Wnt effector TCF-4." in: Nucleic acids research, Vol. 37, Issue 9, pp. 3007-20, 2009 (PubMed).

Bon, Di Carlo, Folgiero et al.: "Negative regulation of beta4 integrin transcription by homeodomain-interacting protein kinase 2 and p53 impairs tumor progression." in: Cancer research, Vol. 69, Issue 14, pp. 5978-86, 2009 (PubMed).

Honys, R?nuak, Fecikovua et al.: "Cytoskeleton-associated large RNP complexes in tobacco male gametophyte (EPPs) are associated with ribosomes and are involved in protein synthesis, processing, and localization." in: Journal of proteome research, Vol. 8, Issue 4, pp. 2015-31, 2009 (PubMed).

Buarta, Vinarskuy, Holubcovua et al.: "Human Embryonic Stem Cells are Capable of Executing G1/S Checkpoint Activation." in: Stem cells (Dayton, Ohio), 2010 (PubMed).

Pospichalova, Tureckova, Fafilek et al.: "Generation of two modified mouse alleles of the Hic1 tumor suppressor gene." in: Genesis (New York, N.Y. : 2000), 2011 (PubMed).

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