alpha Tubulin antibody (TUBA1)

Details for Product anti-TUBA1 Antibody No. ABIN93894
Antigen
Reactivity
All Species
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185
173
40
35
35
25
20
16
15
13
10
9
9
8
8
7
7
7
6
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5
5
5
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4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Host
Mouse
167
132
23
4
2
2
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This alpha Tubulin antibody is un-conjugated
20
9
8
8
8
8
8
8
7
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
264
114
101
89
76
70
52
21
16
13
10
8
3
2
1
1
1
Options
Immunogen Porcine brain microtubule protein MTP-1.
Clone TU-16
Isotype IgM
Specificity The antibody TU-16 reacts with alpha-tubulin of all tested species, under denaturing and non-denaturing conditions.
Cross-Reactivity (Details) Broad species reactivity
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name alpha-tubulin (TUBA1 Antibody Abstract)
Background The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.
Pathways Microtubule Dynamics
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-alpha Tubulin (TUBA1) antibody (ABIN93894) Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16...
Product cited in: Ji, Rath, Girton, Johansen, Johansen: "D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut." in: Journal of neurobiology, Vol. 64, Issue 2, pp. 157-69, 2005 (PubMed).

Qi, Rath, Wang, Xu, Ding, Zhang, Blacketer, Paddy, Girton, Johansen, Johansen: "Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila." in: Molecular biology of the cell, Vol. 15, Issue 11, pp. 4854-65, 2004 (PubMed).

Dráberová, Dráber: "Novel monoclonal antibodies TU-08 and TU-16 specific for tubulin subunits." in: Folia biologica, Vol. 44, Issue 1, pp. 35-6, 2000 (PubMed).