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Details for Product No. ABIN94270

phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) antibody

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Antigen
Synonyms CBP, PAG, Cbp, Pag, F730007C19Rik, MGC80192
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 3 references available
Catalog no. ABIN94270
Quantity 0.1 mg
Price
363.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 4 to 5 Business Days
Immunogen C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Clone PAG-C1
Isotype IgG2b
Specificity The antibody PAG-C1 recognizes an epitope located in the C-terminal domain ofCsk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptorprotein present in membrane rafts (glycosphingolipid-enriched microdomains), whichhowever migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Alternative Name PAG1
Background PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is aubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts(glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gelsanomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src familykinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the majornegative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells orhigh affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosinephosphorylation and Csk binding, while T cell receptor signaling causes PAGdephosphorylation, loss of Csk binding and increased activation of the protein tyrosinekinase Lck.
Application Notes Immunoprecipitation - Positive control: RAJI human Burkitt lymphoma cell line. Immunohistochemistry (paraffin sections) - Positive tissue: Appendix (germinal center of lymphatic follicle. Application note: heat-mediated antigen retrieval in citrate buffer pH 6. 1
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Storage 4 °C
Baumgartner, Angelisová, Setterblad et al.: "Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes." in: Blood, Vol. 101, Issue 5, pp. 1874-81, 2003 (PubMed).

Davidson, Bakinowski, Thomas et al.: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, 2003 (PubMed).

Vang, Abrahamsen, Myklebust et al.: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, 2003 (PubMed).

Alternatives for antigen "phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1)", type "Antibodies"
Hosts (35), (27)
Reactivities (59), (22), (18), (5)
Applications (35), (26), (26), (14), (10), (4)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (17), (9), (1)
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