PAG1 antibody (phosphoprotein Associated with Glycosphingolipid Microdomains 1) (AA 97-432)

Details for Product anti-PAG1 Antibody No. ABIN94497
Antigen
Epitope
AA 97-432
18
14
4
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
68
41
35
16
2
2
1
1
1
Host
Rabbit
50
22
Clonality
Polyclonal
Conjugate
This PAG1 antibody is un-conjugated
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
41
26
20
13
13
9
7
6
1
Options
Immunogen Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).
Specificity The polyclonal antibody recognizes Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Cross-Reactivity (Details) Mouse, Human
Purification Purified from rabbit serum by precipitation methods
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name PAG / Cbp (PAG1 Antibody Abstract)
Background PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Pathways p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 97-432) antibody (ABIN94497) Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)