PAG1 antibody (AA 97-432)

Catalog No. ABIN94497

The Rabbit Polyclonal anti-PAG1 antibody (ABIN94497) specifically detects PAG1 in WB, IP and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for PAG1 antibody (AA 97-432) (ABIN94497)

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PAG1 Antibody

Binding Specificity: AA 97-432

Purpose: Anti-PAG1 Purified

Cross-Reactivity (Details): Mouse, Human

Characteristics: The polyclonal antibody recognizes intracellular part of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Positive tissue: tonsil, colon germinal center.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for PAG1

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Alternative Name: PAG1

Background: Phosphoprotein membrane anchor with glycosphingoli,PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.,CBP, PAG

Gene ID: 94212

UniProt: Q3U1F9

Pathways: p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events