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IL-2 antibody

IL2 Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN964758
Supplier Product No.: 209-401-b95
  • Target See all IL-2 (IL2) Antibodies
    IL-2 (IL2) (Interleukin 2 (IL2))
    Reactivity
    • 141
    • 85
    • 42
    • 13
    • 9
    • 7
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 116
    • 80
    • 58
    • 10
    • 4
    • 2
    • 1
    Rabbit
    Clonality
    • 141
    • 127
    • 1
    Polyclonal
    Conjugate
    • 147
    • 36
    • 24
    • 20
    • 16
    • 9
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL-2 antibody is un-conjugated
    Application
    • 160
    • 130
    • 67
    • 62
    • 40
    • 28
    • 24
    • 21
    • 14
    • 14
    • 13
    • 13
    • 12
    • 11
    • 10
    • 8
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    209-401-b95
    Supplier
    Rockland
    Purpose
    IL-2 Antibody
    Characteristics
    Synonyms: rabbit anti-IL-2 Antibody, rabbit anti-Interleukin-2 antibody, IL-2, IL2, T-cell growth factor, TCGF, Aldesleukin, Lymphokine
    Purification
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.
    Immunogen

    Immunogen: IL-2 antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-2 protein.

    Immunogen Type: Recombinant Protein

    Isotype
    IgG
    Top Product
    Discover our top product IL2 Primary Antibody
  • Application Notes

    Application Note: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 15 kDa in size corresponding to native human IL-2 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: 1:1,000

    ELISA Dilution: 1:10,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: None
    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store Anti-IL-2 antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    IL-2 (IL2) (Interleukin 2 (IL2))
    Alternative Name
    IL2 (IL2 Products)
    Background
    Background: IL-2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. Anti-IL-2 antibody is ideal for investigators involved in Immunology research.
    Gene ID
    3558
    NCBI Accession
    NP_000577
    UniProt
    P60568
    Pathways
    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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