Insulin-Like Growth Factor 1 Receptor (IGF1R) antibody

Antigen: Insulin-Like Growth Factor 1 Receptor (IGF1R)

Synonyms: IGFR, CD221, IGFIR, JTK13, MGC18216, MGC142170, MGC142172, IGFIRC, IGF1R, IGF-1R, DKFZp469I0618, xIGFR, xigf1r, xIGF-1R, hyft, D930020L01, A330103N21Rik

Clonality: Monoclonal

Host: Please enquire

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN966348

Additional Information

Alternative name: IGF1R

Immunogen: Ni-NTA purified truncated recombinant IGF1R-His expressed in E. Coli strain BL21 (DE3)

Isotype: IgG2b

Description: IGF1R(insulin-like growth factor 1 receptor), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation.Insulin receptors (IRs) share significant similarity with IGF1 receptors in both structure and function,including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151).Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.

Application Details

Application Notes: Western Blot: 1: 500- 1: 2,000
IHC(P): 1: 500- 1: 2,000
IHC(F): 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Purification: Crude ascites.

Storage: Store at -20° C.

Restrictions: For Research Use only

Publications

Product: Fujimoto, Tomita, Wakamatsu et al.: "Physical mapping of the conjugative bacteriocin plasmid pPD1 of Enterococcus faecalis and identification of the determinant related to the pheromone response." in: Journal of bacteriology, Vol. 177, Issue 19, pp. 5574-81, 1995 (PubMed).

Scheidegger, Cenni, Picard et al.: "Estradiol decreases IGF-1 and IGF-1 receptor expression in rat aortic smooth muscle cells. Mechanisms for its atheroprotective effects." in: The Journal of biological chemistry, Vol. 275, Issue 49, pp. 38921-8, 2001 (PubMed).