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IGF1R antibody (AA 251-350)

IGF1R Reactivity: Human, Mouse, Rat, Dog FACS Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN726575
  • Target See all IGF1R Antibodies
    IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
    Binding Specificity
    • 26
    • 24
    • 24
    • 13
    • 13
    • 11
    • 10
    • 10
    • 10
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 251-350
    Reactivity
    • 193
    • 116
    • 97
    • 15
    • 7
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Dog
    Host
    • 180
    • 27
    • 2
    • 1
    Rabbit
    Clonality
    • 182
    • 28
    Polyclonal
    Conjugate
    • 119
    • 10
    • 9
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    This IGF1R antibody is un-conjugated
    Application
    • 132
    • 73
    • 61
    • 60
    • 32
    • 30
    • 17
    • 13
    • 13
    • 10
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Cross-Reactivity
    Dog, Human, Mouse, Rat
    Predicted Reactivity
    Cow,Sheep,Pig,Horse,Rabbit
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human IGF-1R alpha chain
    Isotype
    IgG
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Restrictions
    For Research Use only
  • Validation #029750 (Western Blotting)
    'Independent Validation' Badge
    by
    Alamo Laboratories Inc
    No.
    #029750
    Date
    07/01/2014
    Antigen
    Lot Number
    130322
    Method validated
    Western Blotting
    Positive Control
    MCF-7 cells – high expression
    Negative Control
    PC3 cells – low expression
    Notes
    Two strong bands were observed in the positive control, one at the expected molecular weight, and one slightly higher (which may represent a glycosylated or precursor form). Weaker bands of the same molecular weight appear in the negative control, which is consistent with the expected lower expression in PC3 cells. No other major bands are present in either positive or negative controls.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Insulin-Like Growth Factor 1 Receptor (IGF1R) (1:200 dilution)
    • Catalog number: ABIN726575
    • Lot number: 130322
    Secondary Antibody
    • Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
    • Lot number: L170-6515
    Full Protocol
    • Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
    • 46 μg of boiled extracts were loaded and resolved on a 8-16% SDS-polyacrylamide gel.
    • The Spectra Multicolor Broad Range (Thermo Scientific, Cat # 26634) were used as molecular mass markers.
    • Proteins were transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
    • The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) non-fat dry milk at room temperature for 1 h.
    • The membrane was rinsed with TBST once.
    • The membrane was immersed with the protein side up in the primary antibody solution (anti-IGF1R; 1:200) in TBST containing 5% (W/V) non-fat dry milk and incubated for 16 hours at 4°C.
    • The membrane was rinsed in TBST three times for 5 min each.
    • The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) non-fat dry milk and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
    • The membrane was rinsed three times with TBST for 5 min each.
    • The membrane was rinsed in TBS twice for 30 s each.
    • Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
    • The membrane was rinsed three times with TBST, then incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
    • The membrane was washed in TBST 2 times for 10 min each.
    • Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
    Experimental Notes
    - No challenges noted.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Hu, Li, Hu, Wu, Li, Meng: "MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene." in: Growth factors (Chur, Switzerland), Vol. 32, Issue 1, pp. 27-33, (2014) (PubMed).

    Yang, Liu, Li, Wen, Zhu, Xu: "Insulin-Like Growth Factor-1 Modulates Polycomb Cbx8 Expression and Inhibits Colon Cancer Cell Apoptosis." in: Cell biochemistry and biophysics, (2014) (PubMed).

  • Target
    IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
    Alternative Name
    IGF1R (IGF1R Products)
    Synonyms
    CMT2N antibody, CD221 antibody, IGFIR antibody, IGFR antibody, JTK13 antibody, IGFIRC antibody, IGFR1 antibody, IGF-1R antibody, IGF1R antibody, DKFZp469I0618 antibody, igf-1r antibody, xIGF-1R antibody, xIGFR antibody, xigf1r antibody, A330103N21Rik antibody, D930020L01 antibody, hyft antibody, igf-ir antibody, IGF-IR antibody, IGF-IRb antibody, wu:fb03h06 antibody, IGF-IRa antibody, sb:eu1090 antibody, wu:fi20b10 antibody, alanyl-tRNA synthetase antibody, insulin like growth factor 1 receptor antibody, insulin-like growth factor 1 receptor antibody, insulin-like growth factor 1 antibody, insulin-like growth factor I receptor antibody, insulin like growth factor 1 receptor S homeolog antibody, insulin-like growth factor 1b receptor antibody, insulin-like growth factor 1a receptor antibody, AARS antibody, IGF1R antibody, Igf1r antibody, igf1r antibody, Igf1 antibody, LOC780503 antibody, LOC100136427 antibody, igf1r.S antibody, igf1rb antibody, igf1ra antibody
    Background

    Synonyms: IGFR, CD221, IGFIR, JTK13, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, IGF1R

    Background: When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.

    Gene ID
    3480
    UniProt
    P08069
    Pathways
    RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy
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