X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4) antibody

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4)

Synonyms: AW413319, AW545101, 2310057B22Rik, MGC95022, MGC73312, zgc:73312, MGC81443, XRCC4, MGC148661, DKFZp469B0634

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN967264

Additional Information

Alternative name: XRCC4

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-residues of human XRCC4(X-ray repair cross-complementing protein 4)

Description: XRCC4 (X-ray repair cross-complementing protein 4) is involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. XRCC4 binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. XRCC4 is a homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC. XRCC4 seems to interact directly with PRKDC but not with the Ku p70/86 dimer. It interacts with XLF/Cernunnos. Interacts with APTX and APLF. Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. It has no effect on ubiquitination.

Application Details

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Publications

Product: Li, Otevrel, Gao et al.: "The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination." in: Cell, Vol. 83, Issue 7, pp. 1079-89, 1996 (PubMed).

Grawunder, Wilm, Wu et al.: "Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells." in: Nature, Vol. 388, Issue 6641, pp. 492-5, 1997 (PubMed).

Leber, Wise, Mizuta et al.: "The XRCC4 gene product is a target for and interacts with the DNA-dependent protein kinase." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1794-801, 1998 (PubMed).

Nick McElhinny, Snowden, McCarville et al.: "Ku recruits the XRCC4-ligase IV complex to DNA ends." in: Molecular and cellular biology, Vol. 20, Issue 9, pp. 2996-3003, 2000 (PubMed).

Chen, Trujillo, Sung et al.: "Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 26196-205, 2000 (PubMed).