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MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody

Details for Product No. ABIN967314
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Antigen
Synonyms CG11482, Dmel\\CG11482, dmlh-1, dmlh1, zgc:66301, MLH1, LOC100232198, 1110035C23Rik, AI317206, AI325952, AI561766, COCA2, FCC2, HNPCC, HNPCC2, hMLH1
Reactivity
Human
(99), (38), (34), (14), (13), (7), (5), (1), (1), (1), (1), (1)
Host
Mouse
(73), (31)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe))
(72), (30), (29), (17), (10), (10), (10), (9), (8), (6), (2)
Pubmed 7 references available
Quantity 50 µg
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Catalog No. ABIN967314
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Clone G168
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Components 1) 51-1327GR: Purified Mouse Anti-Human MLH-1.
Quantity: 50µg (1 ea).
Concentration: 0.25 mg/ml.
Clone: G168-15.
Immunogen: Human recombinant MLH
Isotype: Mouse IgG1.
Molecular Weight: 80-85 kDa.
Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and Less or equal than 0.09% sodium azide.

2) 51-16526N: Jurkat Cell Lysate.
Quantity: 50µg (1 ea).
Concentration: 1.0 mg/ml.
Alternative Name MLH1
Background The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. In bacteria the DNA repair process is accomplished by the MutL, MutH, and MutS proteins. The MutS protein initially recognizes and binds to mismatched DNA. Following this, MutH, an endonuclease, and MutL form a complex with MutS and carry out an excision repair mechanism. When bacteria are deficient in one of these enzymes a mutator phenotype arises characterized by genetic instability. The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. Biochemical studies of the human homologs of DNA mismatch repair enzymes MLH1, PMS2, and MSH2 indicate that human MSH2 protein can bind mispaired DNA, and that human MLH1 and PMS2 can exist as a heterodimer. These and other studies support the conservation of eukaryotic DNA mismatch repair mechanisms. The G168-15 antibody recognizes human MLH1 (80-85 kDa). Full-length human recombinant MLH was expressed as a fusion protein, affinity purified, and used as immunogen.
Research Area Cancer, DNA/RNA
Application Notes Applications include western blot analysis (0.5-2.0 µg/ml) and immunohistochemical staining of frozen and paraffin-embedded tissue sections (5-20 µg/ml). Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot control (store lysate at -20°C). Additional Jurkat control lysate (ABIN968537) is sold separately. Intestine or normal colon is suggested as a positive control for immunohistochemical staining. In intestine, staining is primarily nuclear and is seen in the crypts of Lieberkuhn, similar to that described in the literature. Both nuclear and cytoplasmic staining have been observed in a variety of other normal and tumor tissue and cell types. Clone G168-728 (ABIN967392) is recommended for immunoprecipitation of MLH1.
Comment

Related Products: ABIN968537, ABIN967392, ABIN967389

Restrictions For Research Use only
Format Liquid
Storage -20 °C
Supplier Images
anti-MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody Western blot analysis of MLH1. Lysate from Jurkat cells were probed with anti-MLH1 (clone G168-15) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). MLH1 is identified as a band between 80-85 kDa.
anti-MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody (2) Acetone-fixed, frozen tissue section of human colon carcinoma stained for MLH1 (clone G168-15) using a DAB chromogen and Hematoxylin counterstain. Cells expressing MLH-1 can be identifed by the intense brown labeling of their cell nuclei.
General Su, Modrich: "Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5057-61, 1986 (PubMed).

Wilson, Ewel, Duguid et al.: "Differential cellular expression of the human MSH2 repair enzyme in small and large intestine." in: Cancer research, Vol. 55, Issue 22, pp. 5146-50, 1995 (PubMed).

Li, Modrich: "Restoration of mismatch repair to nuclear extracts of H6 colorectal tumor cells by a heterodimer of human MutL homologs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 6, pp. 1950-4, 1995 (PubMed).

Prolla, Pang, Alani et al.: "MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast." in: Science (New York, N.Y.), Vol. 265, Issue 5175, pp. 1091-3, 1994 (PubMed).

Prolla, Christie, Liskay: "Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene." in: Molecular and cellular biology, Vol. 14, Issue 1, pp. 407-15, 1994 (PubMed).

Cleaver: "It was a very good year for DNA repair." in: Cell, Vol. 76, Issue 1, pp. 1-4, 1994 (PubMed).

Baker, Plug, Prolla et al.: "Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over." in: Nature genetics, Vol. 13, Issue 3, pp. 336-42, 1996 (PubMed).

Validation Images
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