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MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody

Details for Product No. ABIN967392
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Antigen
Synonyms CG11482, Dmel\\CG11482, dmlh-1, dmlh1, zgc:66301, MLH1, LOC100232198, 1110035C23Rik, AI317206, AI325952, AI561766, COCA2, FCC2, HNPCC, HNPCC2, hMLH1
Reactivity
Human
(99), (38), (34), (14), (13), (7), (5), (1), (1), (1), (1), (1)
Host
Mouse
(73), (31)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP)
(72), (30), (29), (17), (10), (10), (10), (9), (8), (6), (2)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN967392
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Immunogen Recombinant Human MLH
Clone G168-728
Isotype IgG2a
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MLH1
Background The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. Loss of function of DNA repair enzymes can lead to an accumulation of replication errors, resulting in a mutated phenotype. DNA repair enzymes are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex, it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. The G168-728 antibody recognizes human and mouse MLH1 (80-85 kDa). Full-length human recombinant MLH was expressed as a maltose binding-MLH fusion protein, affinity purified, and used as immunogen.
Molecular Weight 80-85 kDa
Research Area Cancer, DNA/RNA
Application Notes Applications include immunoprecipitation (2 µg/1x106 cells) and western blot analysis (1-3 µg/ml). MCF-7 human breast carcinoma (ATCC HTB-22), 293 adenovirustransformed human kidney (ATCC CRL-1673), and NIH/3T3 mouse fibroblast (ATCC CRL-1658) cells are suggested as positive controls. Clone G168-15 is suggested for immunohistochemical analysis of MLH1, clone G168-15 may also be stronger for western blot analysis than clone G168-728 in some assay systems.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody Immunoprecipitation of MLH1. Two different monoclonal antibodies were used to immunoprecipitate MLH1 from equal amounts of whole cell extracts of NIH/3T3 mouse cells. Lane 1, a strong MLH1 band was seen with clone G168-728. Lane 2, only a faint band was seen using clone G168-15. Lane 3, an IgG2a isotype control.
anti-MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody (2) Western blot analysis of MLH1. 30 myg of 293 cell lysate per lane was probed with 3 myg/ml (lane 1) or 1 myg/ml (lane 2) of anti- MLH1 antibody (clone G168-728).
anti-MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody (3) anti-MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody (Image 3)
General Prolla, Pang, Alani et al.: "MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast." in: Science (New York, N.Y.), Vol. 265, Issue 5175, pp. 1091-3, 1994 (PubMed).

Prolla, Christie, Liskay: "Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene." in: Molecular and cellular biology, Vol. 14, Issue 1, pp. 407-15, 1994 (PubMed).

Baker, Plug, Prolla et al.: "Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over." in: Nature genetics, Vol. 13, Issue 3, pp. 336-42, 1996 (PubMed).

Validation Images
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