MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody

Antigen: MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1)

Synonyms: FCC2, COCA2, HNPCC, hMLH1, HNPCC2, MGC5172, MGC66301, zgc:66301, MLH1, MGC140127, LOC100232198

Clonality: Monoclonal (G168-728)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN967392

Additional Information

Alternative name: MLH1

Immunogen: Recombinant Human MLH

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Isotype: IgG2a

Clone: G168-728

Description: The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. Loss of function of DNA repair enzymes can lead to an accumulation of replication errors, resulting in a mutated phenotype. DNA repair enzymes are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex, it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. The G168-728 antibody recognizes human and mouse MLH1 (80-85 kDa). Full-length human recombinant MLH was expressed as a maltose binding-MLH fusion protein, affinity purified, and used as immunogen.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 80-85 kDa

Application Details

Application Notes: Applications include immunoprecipitation (2 µg/1x106 cells) and western blot analysis (1-3 µg/ml). MCF-7 human breast carcinoma (ATCC HTB-22), 293 adenovirustransformed human kidney (ATCC CRL-1673), and NIH/3T3 mouse fibroblast (ATCC CRL-1658) cells are suggested as positive controls. Clone G168-15 is suggested for immunohistochemical analysis of MLH1, clone G168-15 may also be stronger for western blot analysis than clone G168-728 in some assay systems.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4° C.

Research Area: Cancer, DNA/RNA

Restrictions: For Research Use only

Images

Immunoprecipitation of MLH1. Two different monoclonal antibodies were used to immunoprecipitate MLH1 from equal amounts of whole cell extracts of NIH/3T3 mouse cells. Lane 1, a strong MLH1 band was seen with clone G168-728. Lane 2, only a faint band was seen using clone G168-15. Lane 3, an IgG2a isotype control.

Western blot analysis of MLH1. 30 myg of 293 cell lysate per lane was probed with 3 myg/ml (lane 1) or 1 myg/ml (lane 2) of anti- MLH1 antibody (clone G168-728).

anti-MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody (Image 3)

Publications

Prolla, Pang, Alani et al.: "MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast." in: Science (New York, N.Y.), Vol. 265, Issue 5175, pp. 1091-3, 1994 (PubMed).

Prolla, Christie, Liskay: "Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene." in: Molecular and cellular biology, Vol. 14, Issue 1, pp. 407-15, 1994 (PubMed).

Baker, Plug, Prolla et al.: "Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over." in: Nature genetics, Vol. 13, Issue 3, pp. 336-42, 1996 (PubMed).