Friend Leukemia Virus Integration 1 (FLI1) antibody
|Synonyms||EWSR2, SIC-1, FLI1, MGC137447, fli, Xfli, fli1, Xfli1, fli-1, Xfli-1, MGC121593|
Alternatives Western Blotting (WB), Gel Shift (GS), Immunoprecipitation (IP)
|2 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||Fli-1 ets Domain Fusion Protein|
|Description||Fli-1 is a 50 kDa ets-related transcription factor. Like other ets-related proteins it binds to consensus sites in DNA through a 85 amino acid ets domain in the carboxyl region of the protein. Fli-1 is associated with Ewing sarcoma through at (11,22)(q24,q12) chromosomal translocation. This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. Such a translocation is found in 85% of Ewing sarcomas. The resulting fusion protein has the DNA binding activity of Fli-1 and, with the EWS domain, it is also a more potent transcriptional activator than Fli-1 itself. The strong transforming activity of the EWS-Fli-1 fusion protein may in part be due to its ability to trans-activate the promoter for c-myc. C-myc is known to be elevated in Ewing sarcomas. Clone G146-254 was raised against a bacterially expressed Fli-1 ets domain fusion protein.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||50 kDa|
Related Products: ABIN967389, ABIN968537
|Application Notes||Applications include western blot analysis (0.5-2 µg/ml). Additional applications not routinely tested include gel shift and immunoprecipitation. Clone G146-254 has been shown to recognize in vitro translated, recombinant bacterially expressed, and endogenous B cell Fli-1. In gel shift assays this antibody supershifts complexes of Fli-1 and DNA probe.|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Restrictions||For Research Use only|
Bailly, Bosselut, Zucman et al.: "DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma." in: Molecular and cellular biology, Vol. 14, Issue 5, pp. 3230-41, 1994 (PubMed).
May, Lessnick, Braun et al.: "The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1." in: Molecular and cellular biology, Vol. 13, Issue 12, pp. 7393-8, 1994 (PubMed).
|Hosts||Rabbit (62), Mouse (3), Sheep (3)|
|Reactivities||Human (64), Mouse (Murine) (36), Rat (Rattus) (28), Chicken (21), Cow (Bovine) (21), Horse (Equine) (17), Xenopus laevis (3), Zebrafish (3), Dog (Canine) (2), Cat (Feline) (1)|
|Applications||Western Blotting (WB) (46), ELISA (25), Immunofluorescence (IF) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (10), N-Term (7), Internal Region (4), AA 1-453 (1), Center (1), N-Term,Lys67 (1)|