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Friend Leukemia Virus Integration 1 (FLI1) antibody

Details for Product No. ABIN967451
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Antigen
Synonyms FLI1, fli, Xfli, fli1, Xfli1, fli-1, Xfli-1, MGC121593, EWSR2, SIC-1, xlfli, Fli-1, Sic1, cb855, wu:fc45b11
Reactivity
Human
(88), (47), (28), (14), (14), (4), (3), (2), (1), (1)
Host
Mouse
(81), (14), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Gel Shift (GS), Immunoprecipitation (IP)
(81), (31), (24), (17), (14), (9), (9), (3), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN967451
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Immunogen Fli-1 ets Domain Fusion Protein
Clone G146-254
Isotype IgG2a
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Fli-1
Background Fli-1 is a 50 kDa ets-related transcription factor. Like other ets-related proteins it binds to consensus sites in DNA through a 85 amino acid ets domain in the carboxyl region of the protein. Fli-1 is associated with Ewing sarcoma through at (11,22)(q24,q12) chromosomal translocation. This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. Such a translocation is found in 85% of Ewing sarcomas. The resulting fusion protein has the DNA binding activity of Fli-1 and, with the EWS domain, it is also a more potent transcriptional activator than Fli-1 itself. The strong transforming activity of the EWS-Fli-1 fusion protein may in part be due to its ability to trans-activate the promoter for c-myc. C-myc is known to be elevated in Ewing sarcomas. Clone G146-254 was raised against a bacterially expressed Fli-1 ets domain fusion protein.
Molecular Weight 50 kDa
Application Notes Applications include western blot analysis (0.5-2 µg/ml). Additional applications not routinely tested include gel shift and immunoprecipitation. Clone G146-254 has been shown to recognize in vitro translated, recombinant bacterially expressed, and endogenous B cell Fli-1. In gel shift assays this antibody supershifts complexes of Fli-1 and DNA probe.
Comment

Related Products: ABIN967389, ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Friend Leukemia Virus Integration 1 (FLI1) antibody Western blot analysis of Fli-1. Lysate from Jurkat cells was probed with anti-Fli-1 (clone G146-254) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 myg/ml (lane 3). Fli-1 is identified as a band of ~50 kDa.
anti-Friend Leukemia Virus Integration 1 (FLI1) antibody (2) anti-Friend Leukemia Virus Integration 1 (FLI1) antibody (Image 2)
General Bailly, Bosselut, Zucman et al.: "DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma." in: Molecular and cellular biology, Vol. 14, Issue 5, pp. 3230-41, 1994 (PubMed).

May, Lessnick, Braun et al.: "The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1." in: Molecular and cellular biology, Vol. 13, Issue 12, pp. 7393-8, 1994 (PubMed).

Validation Images
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