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FLI1 antibody (N-Term)

FLI1 Reactivity: Human, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3043998
  • Target See all FLI1 Antibodies
    FLI1 (Friend Leukemia Virus Integration 1 (FLI1))
    Binding Specificity
    • 11
    • 9
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 54-73, N-Term
    Reactivity
    • 71
    • 27
    • 18
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    Human, Rat
    Host
    • 49
    • 20
    • 3
    Rabbit
    Clonality
    • 49
    • 23
    Polyclonal
    Conjugate
    • 51
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FLI1 antibody is un-conjugated
    Application
    • 60
    • 28
    • 25
    • 17
    • 15
    • 10
    • 7
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purpose
    Rabbit IgG polyclonal antibody for Friend leukemia integration 1 transcription factor(FLI1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Sequence
    QQEWINQPVR VNVKREYDHM
    Cross-Reactivity (Details)
    Predicted Cross Reactivity: mouse
    No cross reactivity with other proteins.
    Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
    Characteristics
    Rabbit IgG polyclonal antibody for Friend leukemia integration 1 transcription factor(FLI1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Gene Name: Friend leukemia virus integration 1
    Protein Name: Friend leukemia integration 1 transcription factor
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the N-terminus of human FLI1(54-73aa QQEWINQPVRVNVKREYDHM), identical to the related rat and mouse sequences.
    Isotype
    IgG
    Top Product
    Discover our top product FLI1 Primary Antibody
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Preservative
    Thimerosal (Merthiolate), Sodium azide
    Precaution of Use
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Expiry Date
    12 months
  • Choi, El-Hamdi, McGregor: "Endothelial Cell Infection by Guinea Pig Cytomegalovirus Is a Lytic or Persistent Infection Depending on Tissue Origin but Requires Viral Pentamer Complex and pp65 Tegument Protein." in: Journal of virology, Vol. 96, Issue 17, pp. e0083122, (2022) (PubMed).

  • Target
    FLI1 (Friend Leukemia Virus Integration 1 (FLI1))
    Alternative Name
    FLI1 (FLI1 Products)
    Synonyms
    EWSR2 antibody, SIC-1 antibody, FLI1 antibody, fli antibody, Xfli antibody, fli1 antibody, Xfli1 antibody, fli-1 antibody, Xfli-1 antibody, MGC121593 antibody, Fli-1 antibody, Sic1 antibody, cb855 antibody, wu:fc45b11 antibody, xlfli antibody, Fli-1 proto-oncogene, ETS transcription factor antibody, Fli-1 proto-oncogene, ETS transcription factor S homeolog antibody, Friend leukemia integration 1 antibody, Fli-1 proto-oncogene, ETS transcription factor a antibody, Fli-1 proto-oncogene, ETS transcription factor L homeolog antibody, FLI1 antibody, Fli1 antibody, fli1.S antibody, fli1 antibody, fli1a antibody, fli1.L antibody
    Background
    Friend leukemia integration 1 transcription factor, also known as Transcription factor ERGB or Proto-oncogene Fli-1 is a protein that in humans is encoded by the human FLI1 gene. The fli1 protooncogene encodes a member of the ETS family of winged helix-turn-helix transcription factors. This gene is mapped to 11q24.3. Fli-1 is activated through retroviral insertional mutagenesis in 90 % of F-MuLV-induced erythroleukemias. The constitutive activation of fli-1 in erythroblasts leads to a dramatic shift in the Epo/Epo-R signal transduction pathway, blocking erythroid differentiation, activating the Ras pathway, and resulting in massive Epo-independent proliferation of erythroblasts. These results suggest that Fli-1 overexpression in erythroblasts alters their responsiveness to Epo and triggers abnormal proliferation by switching the signaling event(s) associated with terminal differentiation to proliferation.

    Synonyms: ERGB transcription factor antibody|Ewing Sarcoma breakpoint region 2 antibody|EWSR 2 antibody|EWSR2 antibody|FLI 1 antibody|FLI 1 proto oncogene antibody|FLI1 antibody|FLI1 EWS fusion gene antibody|FLI1 proto oncogene antibody|FLI1_HUMAN antibody|Friend leukemia integration 1 transcription factor antibody|Friend leukemia virus integration 1 antibody|Proto-oncogene Fli-1 antibody|SIC 1 antibody|SIC1 antibody|Transcription factor ERGB antibody|Viral integration region FLI1 antibody
    UniProt
    Q01543
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