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Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) antibody

Details for Product No. ABIN967452, Supplier: Log in to see
Antigen
  • 2B1
  • Ab1-021
  • cb1086
  • CPi-23
  • Dis-1
  • Dis1
  • Gm5657
  • OF
  • PU.1
  • pu.1
  • Pu.1
  • pu1
  • Serpina3d
  • Serpina3l
  • Sfpi-1
  • Sfpi1
  • sfpi1
  • SFPI1
  • si:by184l24.2
  • SPI-1
  • Spi-1
  • SPI-A
  • SPI1
  • spi1
  • Spin1
  • Spin2a
  • Tcfpu1
  • Tfpu.1
Reactivity
Human
117
27
21
8
6
6
6
5
5
2
1
1
1
Host
Mouse
91
18
16
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
6
6
6
5
3
3
2
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
95
56
24
18
15
13
3
2
2
2
2
1
Supplier
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Brand BD Pharmingen™
Immunogen PU.1 Fusion Protein
Clone G148-74
Isotype IgG2a
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name PU.1 (SPI1 Antibody Abstract)
Background PU.1 is an ets-related transcription factor expressed in B lymphocytes and macrophages. It was initially identified as the oncogene Spi-1 which was found to block erythroblast differentiation. PU.1 functions by binding transcriptional control elements such as µB in the immunoglobulin heavy chain enhancer and pi adjacent to µE2. It has been shown to form a complex with NF-EM5, a B cell transcription factor, resulting in the activation of the immunoglobulin kappa 3' enhancer. PU.1 also appears to be critically involved in the control of monocyte development by regulating the expression of the macrophage colony-stimulating factor receptor. PU.1 runs as a 40-42 kDa protein on SDS-PAGE. G148-74 recognizes human PU.1, an Ets-related transcription factor. PU.1 is expressed in B lymphocytes and macrophages and like other Ets-related proteins, binds to consensus sites in DNA through an 85 amino acid Ets domain in the carboxyl terminal region of the protein. The antibody was raised against a bacterially expressed GST-PU.1 fusion protein.
Molecular Weight 40-42 kDa
Application Notes Applications include gel shift, western blot analysis (1-2 µg/ml) and immunoprecipitation. The antibody has been shown to recognize in vitro translated PU.1 and PU.1 expressed as a recombinant protein in bacteria. In. In gel shift assays using µB as a probe (µB is a transcriptional element in the immunoglobulin heavy chain enhancer), G148-74 supershifts the complex of probe and PU.1.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Product cited in: Klemsz, McKercher, Celada, Van Beveren, Maki: "Pillars article: the macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene. Cell, 1990. 61: 113-124." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 3, pp. 1597-608, 2008 (PubMed).

Rivera, Stuiver, Steenbergen, Murre: "Ets proteins: new factors that regulate immunoglobulin heavy-chain gene expression." in: Molecular and cellular biology, Vol. 13, Issue 11, pp. 7163-9, 1993 (PubMed).

Shin, Koshland: "Ets-related protein PU.1 regulates expression of the immunoglobulin J-chain gene through a novel Ets-binding element." in: Genes & development, Vol. 7, Issue 10, pp. 2006-15, 1993 (PubMed).

Pongubala, Nagulapalli, Klemsz, McKercher, Maki, Atchison: "PU.1 recruits a second nuclear factor to a site important for immunoglobulin kappa 3' enhancer activity." in: Molecular and cellular biology, Vol. 12, Issue 1, pp. 368-78, 1992 (PubMed).

Moreau-Gachelin, Ray, Mattei, Tambourin, Tavitian: "The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias." in: Oncogene, Vol. 4, Issue 12, pp. 1449-56, 1990 (PubMed).

Background publications Zhang, Hetherington, Chen, Tenen: "The macrophage transcription factor PU.1 directs tissue-specific expression of the macrophage colony-stimulating factor receptor." in: Molecular and cellular biology, Vol. 14, Issue 1, pp. 373-81, 1994 (PubMed).

Klemsz, McKercher, Celada, Van Beveren, Maki: "The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene." in: Cell, Vol. 61, Issue 1, pp. 113-24, 1990 (PubMed).