SPI1 antibody (Internal Region)
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- Target See all SPI1 Antibodies
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This SPI1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- PU.1
- Sequence
- DLYQRQTHEY Y
- Specificity
- This antibody is expected to recognise both reported isoforms (NP_001074016.1, NP_003111.2).
- Cross-Reactivity
- Human, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-DLYQRQTHEYY, from the internal region of the protein sequence according to NP_001074016.1, NP_003111.2.
- Isotype
- IgG
- Top Product
- Discover our top product SPI1 Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Spleen and Colon (lymphoid aggregate). Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 40 kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2 kDa according to NP_001074016.1 and of 31.1 kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with differe
Peptide ELISA: antibody detection limit dilution 1:32000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
- Alternative Name
- SPI1 (SPI1 Products)
- Synonyms
- OF antibody, PU.1 antibody, SFPI1 antibody, SPI-1 antibody, SPI-A antibody, Dis-1 antibody, Dis1 antibody, Sfpi-1 antibody, Sfpi1 antibody, Spi-1 antibody, Tcfpu1 antibody, Tfpu.1 antibody, Pu.1 antibody, cb1086 antibody, pu.1 antibody, pu1 antibody, sfpi1 antibody, si:by184l24.2 antibody, spi1 antibody, SPI1/PU.1 antibody, spi1l antibody, zgc:152991 antibody, Spi-1 proto-oncogene antibody, spleen focus forming virus (SFFV) proviral integration oncogene antibody, Spi-1 proto-oncogene b antibody, Spi-1 proto-oncogene a antibody, SPI1 antibody, Spi1 antibody, spi1b antibody, spi1a antibody
- Background
- PU.1, SPI1, spleen focus forming virus (SFFV) proviral integration oncogene spi1 , hCG_25181, SFPI1, SPI-1, SPI-A, 31 kDa transforming protein, SPI-1 proto-oncogene, hematopoietic transcription factor PU.1
- Gene ID
- 6688, 366126
- NCBI Accession
- NP_001074016, NP_003111
- Pathways
- Stem Cell Maintenance
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