SPI1 antibody (Internal Region)

Catalog No. ABIN334471

This Goat Polyclonal antibody specifically detects SPI1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for SPI1 antibody (Internal Region) (ABIN334471)

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SPI1 Antibody

Binding Specificity: Internal Region

Purpose: PU.1

Sequence: DLYQRQTHEY Y

Specificity: This antibody is expected to recognise both reported isoforms (NP_001074016.1, NP_003111.2).

Cross-Reactivity: Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DLYQRQTHEYY, from the internal region of the protein sequence according to NP_001074016.1, NP_003111.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen and Colon (lymphoid aggregate). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2 kDa according to NP_001074016.1 and of 31.1 kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with differe

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: SPI1

Background: PU.1, SPI1, spleen focus forming virus (SFFV) proviral integration oncogene spi1 , hCG_25181, SFPI1, SPI-1, SPI-A, 31 kDa transforming protein, SPI-1 proto-oncogene, hematopoietic transcription factor PU.1

Gene ID: 6688, 366126

NCBI Accession: NP_001074016, NP_003111

Pathways: Stem Cell Maintenance