SPI1 antibody (AA 16-170)

Catalog No. ABIN6940631

The Mouse Monoclonal anti-SPI1 antibody (Clone PU1-2146) (ABIN6940631) specifically detects SPI1 in WB, IHC, IF, FACS and StM. The antibody is reactive with Human samples.

Quick Overview for SPI1 antibody (AA 16-170) (ABIN6940631)

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: PU1-2146

Product Details anti-SPI1 Antibody

Binding Specificity: AA 16-170

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: Ramos, K-562 cells, THP-1 cells. Lymph Node, Spleen, Hodgkin's Lymphoma, Colon Carcinoma.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: SPI1

Background: PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.

Molecular Weight: 40kDa

Gene ID: 6688

UniProt: P17947

Pathways: Stem Cell Maintenance