Fas (TNFRSF6)-Associated Via Death Domain (FADD) antibody

Antigen: Fas (TNFRSF6)-Associated Via Death Domain (FADD)

Synonyms: GIG3, MORT1, MGC8528, Mort1/FADD

Clonality: Monoclonal (A66-2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN967524

Additional Information

Alternative name: FADD

Immunogen: Human FADD GST

Format: Liquid

Isotype: IgG1

Clone: A66-2

Description: FADD is a molecule involved in the Fas-mediated cell death pathway. Apoptosis is induced when Fas ligand or agonistic Fas antibodies bind to the Fas receptor, and trigger the activation of a cell death signaling pathway. Induction of Fas-mediated apoptosis requires a conserved cytoplasmic motif, referred to as the death domain, that is present in the C-terminal end of Fas. FADD also contains a death domain, and Fas and FADD bind to each other through their respective death domains. Death domains are thought to act as adaptor proteins by linking Fas and other members of the tumor necrosis factor receptor (TNFR) superfamily to downstream signaling pathways. Overexpression of FADD in vitro leads to cell death suggesting that FADD, like FAS, is an apoptosis-inducing protein. The N-terminal, but not the C-terminal death domain, is required for apoptosis induced by FADD overexpression. It is thought that the amino-terminal region of FADD functions by binding to caspase-3 and thereby linking signals from the cell surface to an apoptopic protease cascade. FADD has a calculated molecular weight of 24 kDa and migrates at a molecular weight of ~27 kDa in SDS/PAGE.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 24-27 kDa

Comments:

Related Products: ABIN967389

Application Details

Application Notes: Clone A66-2 can be used for western blot analysis (1-2 µg/ml). Other reported applications not routinely tested include immunoprecipitation (1-2 µg/1x10^6 cells). Daudi B lymphoma cells (ATCC CCL-213) are suggested as a positive control.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Cancer, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of FADD. Daudi B lymphoma cell lysate was probed with anti-human FADD (clone A66-2) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). The antibody identifies FADD as an ~27 kDa band.

anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) antibody (Image 2)

Publications

Product: Cleveland, Ihle: "Contenders in FasL/TNF death signaling." in: Cell, Vol. 81, Issue 4, pp. 479-82, 1995 (PubMed).

Muzio, Chinnaiyan, Kischkel et al.: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996 (PubMed).