Images for product: anti-Linker For Activation of T Cells (LAT) (pTyr226) antibody
Western blot analysis of LAT (pY226) in human T lymphocytes. Lysates from control (lanes 1-3) and activated (anti-CD3, plus anti-CD28, lanes 4-6) Jurkat T-cell leukemia were probed with purified mAb J96-1238.58.93 at concentrations of 1.0 (lanes 1 and 4), 0.5 (lanes 2 and 5), and 0.025 µg/ml (lanes 3 and 6). LAT (pY226) is identified as a band of 38 kDa in the treated cells.
LAT (pY226) staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer, the sections were either left untreated (First Panel) or treated with a phosphatase to eliminate all phosphorylation (Second Panel). The tissue sections were stained with purified Mouse anti-LAT (pY226) with Hematoxylin counterstaining. Original magnification: 20X.