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SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody

Details for Product No. ABIN967676
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Antigen
Synonyms ANOP3, MCOPS3, RGD1565646, Qsox, rSOx, Qscn6, Sox-2, cb236, wu:fb83g04, wu:fc14d07, zgc:65860, zgc:77389, SRY-box2, Xsox-2, anop3, mcops3, sox-2
Reactivity
Human
(185), (65), (44), (21), (20), (20), (16), (12), (5), (4), (4), (4), (2), (2), (1), (1), (1)
Host
Mouse
(154), (39), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (6), (6), (6), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Intracellular Flow Cytometry (ICFC), BioImaging (BI), Immunofluorescence (IF)
(140), (73), (36), (33), (31), (29), (26), (18), (10), (7), (2), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN967676
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Immunogen Human Sox2 Recombinant Protein
Clone O30-678
Isotype IgG1, kappa
Cross-Reactivity Mouse (Murine)
Characteristics 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. mTESR™1 is a trademark of StemCell Technologies.
5. Triton is a trademark of the Dow Chemical Company.
6. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name SOX2
Background The monoclonal antibody O30-678 recognizes the Sox2 transcription factor. Sox2 [SRY (sex determining region Y)-box 2] is a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox2 is required for the maintenance of the undifferentiated state of pluripotent stem cells. Complexes of Sox2 with the homeobox transcription factors Oct3/4 and/or Nanog bind to the promoters of a network of genes that are involved in the maintenance of pluripotency and self renewal in stem cells. Sox2 is also a marker of neural stem cells during embryonic development and in the adult brain. The O30-678 antibody recognizes both human and mouse Sox2 proteins.
Molecular Weight 36 kDa
Gene ID 6657, 20674
Research Area Embryogenesis, Embryonic stem cells, Reproduction, Transcription Factors, Neural Stem Cell marker
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody Western Blot analysis of Sox2 in Human Neural Stem Cells (NSC) and Mouse Embryonic Stem (ES) cell line. Lysates of NSC derived from H9 human ES cells (WiCell, Madison, WI, Left) and ES-E14TG2a mouse ES cells (ATCC CRL-1821, right) were probed with Purified Mouse anti-Sox2 monoclonal antibody at concentrations of 0.125 (lanes 1), 0.06 (lanes 2), and 0.03 myg/ml (lanes 3). Human and mouse Sox2 were identified as bands of ~36 kDa.
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (2) Immunoflourescent staining of Sox2 in human embryonic stem (ES) cells. H9 human ES cells (WiCell Madison, WI), passage 52 grown in mTESR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix, were fixed with BD Cytofix™ Fixation Buffer, permeabilized with BD™ Phosflow Perm Buffer III (Cat No. 558050), and stained with Purified Mouse anti-Sox2 monoclonal antibody (pseudo colored green) at 10 mg/mL. The second step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. Permeabilization with BD Perm/Wash™ (Cat No. 554723) or 0.1% Triton™ X-100 buffer is also suitable for use with this antibody.
Product cited in: Boyer, Lee, Cole et al.: "Core transcriptional regulatory circuitry in human embryonic stem cells." in: Cell, Vol. 122, Issue 6, pp. 947-56, 2005 (PubMed).

Takahashi, Yamanaka: "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors." in: Cell, Vol. 126, Issue 4, pp. 663-76, 2006 (PubMed).

Pan, Thomson: "Nanog and transcriptional networks in embryonic stem cell pluripotency." in: Cell research, Vol. 17, Issue 1, pp. 42-9, 2007 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967676
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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