You are viewing an incomplete version of our website. Please click to reload the website as full version.

B-Cell CLL/lymphoma 6 (BCL6) antibody

Details for Product No. ABIN967677, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms bcl5, laz3, bcl6a, znf51, zbtb27, MGC132260, Bcl5, BCL5, BCL6A, LAZ3, ZBTB27, ZNF51
Human, Mouse (Murine)
(154), (76), (45), (9), (3), (3), (1), (1), (1), (1)
(99), (51), (7), (1)
Clonality (Clone)
Monoclonal ()
(9), (8), (7), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Intracellular Staining (ICS), Western Blotting (WB)
(104), (60), (32), (28), (21), (19), (17), (11), (5), (2), (2), (1), (1), (1), (1)
Pubmed 10 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 0.1 mg
Shipping to United States ( )
Brand BD Pharmingen™
Immunogen Human Bcl-6 Recombinant Protein
Clone K112-91
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. This product is sold under license to the following patent: US Patent No. 6,174,99
6. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name BCL-6 (BCL6 Antibody Abstract)
Background The K112-91 monoclonal antibody specifically binds to Bcl-6. Bcl-6 was first identified as a proto-oncogene frequently deregulated by chromosomal translocations in non-Hodgkin B-cell lymphomas. It is a nuclear transcriptional repressor of the BTB/POZ zinc-finger family of transcription factors. In addition to its role in cancer, Bcl-6 plays an important role in normal lymphocyte differentiation. Bcl-6 is highly expressed in germinal center B cells, where it promotes the germinal center reaction by inducing proliferation and inhibiting the DNA-damage response. Additionally, Bcl-6 has been identified as a key factor in promoting the differentiation of CD4+ follicular T helper (Tfh) cells, which are involved in promoting germinal center formation and providing help to B cells. The interplay of Bcl-6 and another transcriptional repressor, Blimp-1, is thought to be critical in defining the results of both B-cell and T-cell differentiation.
Synonyms: BCL6, B-cell lymphoma 6 protein, LAZ3, Laz-3, ZBTB27, ZNF51
Molecular Weight 87–98 kDa
Gene ID 604, 12053

Related Products: ABIN967389, ABIN968903, ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 6 (BCL6) antibody (ABIN967677) Analysis of Bcl-6 expression by Western blot and Immunohistochemistry. Western blot a...
Product cited in: Baumjohann, Okada, Ansel: "Cutting Edge: Distinct waves of BCL6 expression during T follicular helper cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 5, pp. 2089-92, 2011 (PubMed).

Choi, Kageyama, Eto et al.: "ICOS receptor instructs T follicular helper cell versus effector cell differentiation via induction of the transcriptional repressor Bcl6." in: Immunity, Vol. 34, Issue 6, pp. 932-46, 2011 (PubMed).

Crotty: "Follicular helper CD4 T cells (TFH)." in: Annual review of immunology, Vol. 29, pp. 621-63, 2011 (PubMed).

Crotty, Johnston, Schoenberger: "Effectors and memories: Bcl-6 and Blimp-1 in T and B lymphocyte differentiation." in: Nature immunology, Vol. 11, Issue 2, pp. 114-20, 2010 (PubMed).

Nurieva, Chung, Martinez et al.: "Bcl6 mediates the development of T follicular helper cells." in: Science (New York, N.Y.), Vol. 325, Issue 5943, pp. 1001-5, 2009 (PubMed).

Johnston, Poholek, DiToro et al.: "Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation." in: Science (New York, N.Y.), Vol. 325, Issue 5943, pp. 1006-10, 2009 (PubMed).

Fazilleau, McHeyzer-Williams, Rosen et al.: "The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding." in: Nature immunology, Vol. 10, Issue 4, pp. 375-84, 2009 (PubMed).

Klein, Dalla-Favera: "Germinal centres: role in B-cell physiology and malignancy." in: Nature reviews. Immunology, Vol. 8, Issue 1, pp. 22-33, 2007 (PubMed).

Ye, Lista, Lo Coco et al.: "Alterations of a zinc finger-encoding gene, BCL-6, in diffuse large-cell lymphoma." in: Science (New York, N.Y.), Vol. 262, Issue 5134, pp. 747-50, 1993 (PubMed).

Background publications Steffen, MacKay, Kerr et al.: "Yeast life span extension by depletion of 60s ribosomal subunits is mediated by Gcn4." in: Cell, Vol. 133, Issue 2, pp. 292-302, 2008 (PubMed).

Catalog No. ABIN967677
Contact our Customer Service for availability and price in your country.

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)