B-Cell CLL/lymphoma 6 (BCL6) antibody

Antigen: B-Cell CLL/lymphoma 6 (BCL6)

Synonyms: BCL5, LAZ3, BCL6A, ZNF51, ZBTB27, bcl5, laz3, bcl6a, znf51, zbtb27, MGC132260, BCL6, DKFZp468I1913

Clonality: Monoclonal (K112-91)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Intracellular Flow Cytometry (ICFC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe))

Catalog no.: ABIN967677

Additional Information

Alternative name: BCL-6

Gene ID: 604, 12053

Immunogen: Human Bcl-6 Recombinant Protein

Format: Liquid

Isotype: IgG1, kappa

Clone: K112-91

Description: The K112-91 monoclonal antibody specifically binds to Bcl-6. Bcl-6 was first identified as a proto-oncogene frequently deregulated by chromosomal translocations in non-Hodgkin B-cell lymphomas. It is a nuclear transcriptional repressor of the BTB/POZ zinc-finger family of transcription factors. In addition to its role in cancer, Bcl-6 plays an important role in normal lymphocyte differentiation. Bcl-6 is highly expressed in germinal center B cells, where it promotes the germinal center reaction by inducing proliferation and inhibiting the DNA-damage response. Additionally, Bcl-6 has been identified as a key factor in promoting the differentiation of CD4+ follicular T helper (Tfh) cells, which are involved in promoting germinal center formation and providing help to B cells. The interplay of Bcl-6 and another transcriptional repressor, Blimp-1, is thought to be critical in defining the results of both B-cell and T-cell differentiation.
Synonyms: BCL6, B-cell lymphoma 6 protein, LAZ3, Laz-3, ZBTB27, ZNF51

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. This product is sold under license to the following patent: US Patent No. 6,174,99
6. Please refer to us for technical protocols.

Molecular Weight: 87–98 kDa

Comments:

Related Products: ABIN967389, ABIN968903, ABIN968537

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Images

Analysis of Bcl-6 expression by Western blot and Immunohistochemistry. Western blot analysis of Bcl-6 expressed by Human Jurkat (ABIN968537) and Ramos cell lines. Cell lysates from untreated Jurkat (lanes 1-5) and Ramos (lanes 6-10) cells (15 µg total cellular protein/lane) were electrophoresed (SDS-PAGE), transferred to membranes and then probed with Purified Mouse Anti-Bcl-6 antibody (Clone K112-91, ABIN967677) at concentrations of 2 (lanes 1, 6), 0.667 (lanes 2, 7), 0.222 (lanes 3, 8), 0.074 (lanes 4, 9) and 0.025 (lanes 5, 10) µg/ml. Bcl-6 is identified as a band of ~87-98 kDa in the Ramos cell lysate.

Publications

Steffen, MacKay, Kerr et al.: "Yeast life span extension by depletion of 60s ribosomal subunits is mediated by Gcn4." in: Cell, Vol. 133, Issue 2, pp. 292-302, 2008 (PubMed).

Product: Ye, Lista, Lo Coco et al.: "Alterations of a zinc finger-encoding gene, BCL-6, in diffuse large-cell lymphoma." in: Science (New York, N.Y.), Vol. 262, Issue 5134, pp. 747-50, 1993 (PubMed).

Klein, Dalla-Favera: "Germinal centres: role in B-cell physiology and malignancy." in: Nature reviews. Immunology, Vol. 8, Issue 1, pp. 22-33, 2007 (PubMed).

Fazilleau, McHeyzer-Williams, Rosen et al.: "The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding." in: Nature immunology, Vol. 10, Issue 4, pp. 375-84, 2009 (PubMed).

Johnston, Poholek, DiToro et al.: "Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation." in: Science (New York, N.Y.), Vol. 325, Issue 5943, pp. 1006-10, 2009 (PubMed).

Nurieva, Chung, Martinez et al.: "Bcl6 mediates the development of T follicular helper cells." in: Science (New York, N.Y.), Vol. 325, Issue 5943, pp. 1001-5, 2009 (PubMed).

Crotty, Johnston, Schoenberger: "Effectors and memories: Bcl-6 and Blimp-1 in T and B lymphocyte differentiation." in: Nature immunology, Vol. 11, Issue 2, pp. 114-20, 2010 (PubMed).

Crotty: "Follicular helper CD4 T cells (TFH)." in: Annual review of immunology, Vol. 29, pp. 621-63, 2011 (PubMed).

Choi, Kageyama, Eto et al.: "ICOS receptor instructs T follicular helper cell versus effector cell differentiation via induction of the transcriptional repressor Bcl6." in: Immunity, Vol. 34, Issue 6, pp. 932-46, 2011 (PubMed).

Baumjohann, Okada, Ansel: "Cutting Edge: Distinct waves of BCL6 expression during T follicular helper cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 5, pp. 2089-92, 2011 (PubMed).