Gap Junction Protein, alpha 1, 43kDa (GJA1) (AA 252-270) antibody

Antigen: Gap Junction Protein, alpha 1, 43kDa (GJA1)

Synonyms: CX43, GJAL, ODDD, DFNB38, MGC128721, gja1-A, connexin 43, cx43, gjal, oddd, dfnb38, connexin43, Cx43

Binding Site: AA 252-270

Clonality: Monoclonal (2)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN967717

Additional Information

Alternative name: Connexin-43

Immunogen: Rat Connexin-43

Cross-Reactivity: Chicken, Dog (Canine), Human, Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 2

Description: Gap junctions are intercellular protein pores. They enable cell-to-cell communication by allowing passage of ions and other small molecules. The subunits of gap junction channels are assembled from a family of proteins called connexins. Individual connexin molecules join to make hexameric hemichannels termed connexons, these structures dock to connexons on neighboring cells, forming gap junction pores. Connexin-43 is a member of the connexin family possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. In addition, it is believed that phosphorylation of connexin-43 plays a regulatory role both in the assembly of connexons and in gating activity at the gap junction. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 43 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: Extracellular Matrix, Cytoskeleton, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of Connexin-43 on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- Connexin-43 antibody.

Immunofluorescence staining on WI38 cells (human lung).

Publications

Product: Loo, Berestecky, Kanemitsu et al.: "pp60src-mediated phosphorylation of connexin 43, a gap junction protein." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12751-61, 1995 (PubMed).

Laird, Castillo, Kasprzak: "Gap junction turnover, intracellular trafficking, and phosphorylation of connexin43 in brefeldin A-treated rat mammary tumor cells." in: The Journal of cell biology, Vol. 131, Issue 5, pp. 1193-203, 1996 (PubMed).

Lucke, Choudhry, Thom et al.: "Upregulation of connexin 26 is a feature of keratinocyte differentiation in hyperproliferative epidermis, vaginal epithelium, and buccal epithelium." in: The Journal of investigative dermatology, Vol. 112, Issue 3, pp. 354-61, 1999 (PubMed).

Toyofuku, Akamatsu, Zhang et al.: "c-Src regulates the interaction between connexin-43 and ZO-1 in cardiac myocytes." in: The Journal of biological chemistry, Vol. 276, Issue 3, pp. 1780-8, 2001 (PubMed).

Giepmans, Hengeveld, Postma et al.: "Interaction of c-Src with gap junction protein connexin-43. Role in the regulation of cell-cell communication." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 8544-9, 2001 (PubMed).