Connexin 43/GJA1 antibody (N-Term)

Catalog No. ABIN2774853

The Rabbit Polyclonal anti-Connexin 43/GJA1 antibody (ABIN2774853) specifically detects Connexin 43/GJA1 in WB. The antibody is reactive with Human, Rat, Mouse, Dog, Cow, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Horse and Sheep samples.

Quick Overview for Connexin 43/GJA1 antibody (N-Term) (ABIN2774853)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Horse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: N-Term

Purpose: GJA1 Antibody - N-terminal region

Sequence: LYLAHVFYVM RKEEKLNKKE EELKVAQTDG VNVDMHLKQI EIKKFKYGIE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against GJA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GJA1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8101310

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: GJA1

Background:

Background Information: Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.

Gene Name: Gap junction protein, alpha 1, 43 kDa

Alternative Symbols: HSS, CMDR, CX43, EKVP, GJAL, ODDD, AVSD3, EKVP3, HLHS1, PPKCA

Protein Name: Gap junction protein RuleBase RU000630

Molecular Weight: 42kDa

Gene ID: 2697

NCBI Accession: NP_000156

UniProt: Q53FJ6

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization