Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 156-322) antibody

Antigen: Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2)

Synonyms: TRKB, GP145-TrkB, Tkrb, trkB, AI848316, C030027L06Rik, TRKB1, RATTRKB1, trkb, xtrkb, trkb-b, MGC52547, NTRK2, Trk-B, MGC140769, ntrk2b, ntrk2, MGC99292, MGC145900, gp145-trkb

Binding Site: AA 156-322

Clonality: Monoclonal (47)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), BioImaging (BI), Immunofluorescence (IF)

Catalog no.: ABIN967745

Additional Information

Alternative name: TrkB

Immunogen: Human TrkB

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 47

Description: The full-length TrkB gene has been reported to encode for a 145 kDa glycosylated transmembrane tyrosine kinase and neurotrophin receptor. The same gene also has been reported to encode for a 95 kDa glycoprotein that is identical to gp145 [TrkB] at the extracellular domain and transmembrane portion but lacks the intracellular portion. TrkB has been observable in a range of 116-145 kD and 70-95 kD due to various TrkB maturation states, subcellular localizations, and glycosylation states. TrkB belongs to a family of tyrosine kinases that include the TrkA proto-oncogene and TrkC. All have an extracellular ligand-binding domain, a transmembrane region, and intracellular kinase and autophosphorylation domains. TrkB binds the neurotrophins NT3 and NT4/5, as well as brain-derived neurotrophic factor (BDNF), a peptide that helps motor neuron survival and repair. The TrkB tyrosine kinase is activated upon binding to BDNF resulting in autophosphorylation of residues Y670, Y674 and Y675 and the subsequent association of several intracellular proteins like PLCgamma, Shc, and PI3-Kinase. TrkB is widely expressed in cells of neuroepithelium and neural crest origin. Some of these include motor neurons, dopamine-producing neurons, and neurons which release gamma-aminobutyric acid in the substantia nigra, neocortex, and hippocampus. The two TrkB gene products are differentially expressed in regions of the adult brain.
Synonyms: Neurotrophic Tyrosine Kinase Receptor Type 2, NTRK2

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 116-145 & 70-95 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Cancer

Restrictions: For Research Use only

Images

Western blot analysis of TrkB on a rat cerebrum lysate (left). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-TrkB antibody.

Immunofluorescent staining of undifferentiated (left) and differentiated (right) SH-SY5Y cells (ATCC CRL-2266, Human neuroblastoma). Undifferentiated cells were seeded in a collagen coated 384 well imaging plate at ~ 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol (see Recommended Assay Procedure, Bioimaging protocol link) and the mouse anti-Trk B antibody. Differentiated cells were seeded in a 96 well, collagen coated imaging plate at ~ 5,000 cells per well. Cells were incubated with 50 mM ATRA (Sigma-Aldrich, R2625) for 5 days, followed by 50 ng/ml BDNF (Sigma-Aldrich, B3795) for 5 days. Differentiated cells were fixed and stained using the methanol fix/perm protocol, and the mouse anti-Trk B antibody. The second step reagent in both cases was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen) (pseudo colored green) and counter-stained with Hoechst 33342 (pseudo colored blue). The images were taken on a BD Pathway™ 855 or 435 imager, using a 20x objective and merged using BD AttoVision™ software. This antibody also stained undifferentiated SK-N-SH cells (ATCC HTB-11, Human neuroblastoma) and C6 cells (ATCC CCL-107, Rat glial cells) using both the Triton X100 and methanol fix/perm protocols (see Recommended Assay Procedure, Bioimaging protocol link).

Immunofluorescent staining of differentiated SH-SY5Y cells

Publications

Product: Ebendal: "Function and evolution in the NGF family and its receptors." in: Journal of neuroscience research, Vol. 32, Issue 4, pp. 461-70, 1992 (PubMed).

Klein, Conway, Parada et al.: "The trkB tyrosine protein kinase gene codes for a second neurogenic receptor that lacks the catalytic kinase domain." in: Cell, Vol. 61, Issue 4, pp. 647-56, 1990 (PubMed).

Armanini, McMahon, Sutherland et al.: "Truncated and catalytic isoforms of trkB are co-expressed in neurons of rat and mouse CNS." in: The European journal of neuroscience, Vol. 7, Issue 6, pp. 1403-9, 1995 (PubMed).

Kryl, Yacoubian, Haapasalo et al.: "Subcellular localization of full-length and truncated Trk receptor isoforms in polarized neurons and epithelial cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 14, pp. 5823-33, 1999 (PubMed).

Jovanovic, Czernik, Fienberg et al.: "Synapsins as mediators of BDNF-enhanced neurotransmitter release." in: Nature neuroscience, Vol. 3, Issue 4, pp. 323-9, 2000 (PubMed).

Muller, Djebbara-Hannas, Jourdain et al.: "Brain-derived neurotrophic factor restores long-term potentiation in polysialic acid-neural cell adhesion molecule-deficient hippocampus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 8, pp. 4315-20, 2000 (PubMed).

Du, Feng, Yang et al.: "Activity- and Ca(2+)-dependent modulation of surface expression of brain-derived neurotrophic factor receptors in hippocampal neurons." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1423-34, 2000 (PubMed).