anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (AA 1-110)

Details for Product anti-MAP2K2 Antibody No. ABIN967846
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Synonyms Prkmk2, CFC4, MAPKK2, MEK2, MKK2, PRKMK2, AA589381, MK2, ATMKK2, F27B13.50, F27B13_50, MAP KINASE KINASE 1, MAP kinase kinase 2, MK1
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AA 1-110
(46), (24), (15), (14), (13), (12), (11), (10), (8), (7), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(256), (125), (104), (10), (5), (2), (2), (1), (1)
(207), (47), (9), (1)
Clonality (Clone)
Monoclonal ()
This MAP2K2 antibody is un-conjugated
(11), (11), (10), (7), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3)
Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
(212), (86), (78), (49), (39), (30), (26), (18), (15), (15), (10), (9), (4), (2), (1), (1)
Pubmed 5 references available
Quantity 150 μg
Shipping to United States ( )
Immunogen Rat MEK2 aa. 1-110
Clone 96-MEK2
Isotype IgG2a
Cross-Reactivity Mouse (Murine), Chicken, Dog (Canine), Frog, Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MEK2 (MAP2K2 Antibody Abstract)
Background MEK2 (MAP Kinase Kinase 2 or ERK Kinase), a 46 kDa protein kinase, phosphorylates MAP kinases (ERKs) at tyrosine and threonine residues. This phosphorylation results in activation of the MAP kinases. MEK2 is seven amino acids larger and shares 81% identity with MEK1. In cultured cells, MEK2 is activated by serum. In vitro, v-Raf phosphorylates and activates MEK2. It is thought that all of these activated protein kinases are downstream of the Ras signal transduction pathway and represent an integral part of the Ras mitogenic signal. This antibody is routinely tested by western blot analysis.
Synonyms: MAP Kinase Kinase 2, ERK Kinase
Molecular Weight 46 kDa
Research Area Cancer, Tyrosine Kinases, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (AA 1-110) (ABIN967846) Western blot analysis of MEK2 on a RSV-3T3 cell lysate. Lane 1: 1:2500, lane 2: 1:500...
Immunofluorescence (IF) image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (AA 1-110) (ABIN967846) Immunofluorescence staining of human fibroblasts.
Image no. 3 for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (AA 1-110) (ABIN967846) anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-110) antibody (Image 3)
Product cited in: Tworkowski, Salghetti, Tansey: "Stable and unstable pools of Myc protein exist in human cells." in: Oncogene, Vol. 21, Issue 55, pp. 8515-20, 2002 (PubMed).

Downey, Butler, Tapper et al.: "Importance of MEK in neutrophil microbicidal responsiveness." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 1, pp. 434-43, 1998 (PubMed).

Wu, Harrison, Dent et al.: "Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4539-48, 1993 (PubMed).

Hattori, Fukuda, Yamashita et al.: "Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system." in: The Journal of biological chemistry, Vol. 267, Issue 28, pp. 20346-51, 1992 (PubMed).

Background publications Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Catalog No. ABIN967846
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