Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-110) antibody

Details for Product No. ABIN967846
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Antigen
Synonyms Prkmk2, CFC4, MAPKK2, MEK2, MKK2, PRKMK2, AA589381, MK2, ATMKK2, F27B13.50, F27B13_50, MAP KINASE KINASE 1, MAP kinase kinase 2, MK1
Epitope
AA 1-110
(41), (15), (12), (9), (8), (7), (7), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(182), (95), (86), (13), (3), (2), (1)
Host
Mouse
(153), (30), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
(145), (60), (56), (38), (34), (30), (25), (13), (10), (8), (8), (4), (4), (1), (1)
Pubmed 5 references available
Quantity 150 μg
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Catalog No. ABIN967846
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Immunogen Rat MEK2
Clone COL 94
Isotype IgG2a
Cross-Reactivity Mouse (Murine), Chicken, Dog (Canine), Frog, Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MEK2
Background MEK2 (MAP Kinase Kinase 2 or ERK Kinase), a 46 kDa protein kinase, phosphorylates MAP kinases (ERKs) at tyrosine and threonine residues. This phosphorylation results in activation of the MAP kinases. MEK2 is seven amino acids larger and shares 81% identity with MEK1. In cultured cells, MEK2 is activated by serum. In vitro, v-Raf phosphorylates and activates MEK2. It is thought that all of these activated protein kinases are downstream of the Ras signal transduction pathway and represent an integral part of the Ras mitogenic signal. This antibody is routinely tested by western blot analysis.
Synonyms: MAP Kinase Kinase 2, ERK Kinase
Molecular Weight 46 kDa
Research Area Cancer, Tyrosine Kinases, Kinases/Phosphatases
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-110) antibody Immunofluorescence staining of human fibroblasts.
anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-110) antibody (2) Western blot analysis of MEK2 on a RSV-3T3 cell lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of the anti- MEK2 antibody.
anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-110) antibody (3) anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-110) antibody (Image 3)
Product cited in: Hattori, Fukuda, Yamashita et al.: "Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system." in: The Journal of biological chemistry, Vol. 267, Issue 28, pp. 20346-51, 1992 (PubMed).

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Wu, Harrison, Dent et al.: "Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4539-48, 1993 (PubMed).

Downey, Butler, Tapper et al.: "Importance of MEK in neutrophil microbicidal responsiveness." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 1, pp. 434-43, 1998 (PubMed).

Tworkowski, Salghetti, Tansey: "Stable and unstable pools of Myc protein exist in human cells." in: Oncogene, Vol. 21, Issue 55, pp. 8515-20, 2002 (PubMed).

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