RAN, Member RAS Oncogene Family (RAN) (AA 7-171) antibody

Antigen: RAN, Member RAS Oncogene Family (RAN)

Synonyms: TC4, Gsp1, ARA24, ran-1, ran, MGC53579, RAN, tc4, gsp1, ara24, MGC69330, MGC127753, DKFZp459G1118, DKFZp468B1019, xran

Binding Site: AA 7-171

Clonality: Monoclonal (20)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN967913

Additional Information

Alternative name: Ran

Immunogen: Human Ran

Cross-Reactivity: Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG2a

Clone: 20

Description: Ran is a highly conserved GTPase that is ubiquitously expressed. At steady-state, 80-90% of cellular Ran is located in the nucleus, with the remainder in the cytoplasm. The human TC4/Ran cDNA was originally isolated based on the predicted homology of its encoded protein product to Ras. Unlike other members of the Ras superfamily, Ran does not appear to contain signals for lipid modification, nor does it appear to show membrane localization. Ran has been implicated in a number of cellular processes such as the initiation of DNA replication, entry into and exit from mitosis and in nuclear RNA and protein transport through the nuclear pore complex. A number of Ran binding proteins have been identified. One of the smaller proteins identified is a 28kDa cytosolic molecule known as RanBP1. The GTP-bound state of Ran is stabilized through its binding to RanBP1.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 25 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Chromatin and Nuclear Signaling

Restrictions: For Research Use only

Images

Western blot analysis of Ran on A431 cell lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-Ran antibody.

Immunofluorescent staining of HeLa cells.

anti-RAN, Member RAS Oncogene Family (RAN) (AA 7-171) antibody (Image 3)

Publications

Product: Drivas, Shih, Coutavas et al.: "Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line." in: Molecular and cellular biology, Vol. 10, Issue 4, pp. 1793-8, 1990 (PubMed).

Iborra, Jackson, Cook: "The path of RNA through nuclear pores: apparent entry from the sides into specialized pores." in: Journal of cell science, Vol. 113 Pt 2, pp. 291-302, 2000 (PubMed).

Faleiro, Lazebnik: "Caspases disrupt the nuclear-cytoplasmic barrier." in: The Journal of cell biology, Vol. 151, Issue 5, pp. 951-9, 2000 (PubMed).

Nemergut, Lindsay, Brownawell et al.: "Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor." in: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 17385-8, 2002 (PubMed).

Schwoebel, Ho, Moore: "The mechanism of inhibition of Ran-dependent nuclear transport by cellular ATP depletion." in: The Journal of cell biology, Vol. 157, Issue 6, pp. 963-74, 2002 (PubMed).