anti-SMARCA2 antibody (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2) (AA 1400-1586)

Details for Product anti-SMARCA2 Antibody No. ABIN967938
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Synonyms BAF190, BRM, NCBRS, SNF2, SNF2L2, SNF2LA, SWI2, Sth1p, hBRM, hSNF2a, brm, 2610209L14Rik, SNF2alpha, Snf2l2, brahma, psa-4, wu:fa56c07, wu:fi27f11, zgc:66238, Brahma
AA 1400-1586
(6), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(43), (13), (11), (4), (3), (3), (2), (2), (1), (1)
(36), (5), (2)
Clonality (Clone)
Monoclonal ()
This SMARCA2 antibody is un-conjugated
(1), (1), (1)
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(38), (16), (11), (7), (7), (3), (2)
Pubmed 3 references available
Quantity 50 μg
Shipping to United States ( )
Immunogen Human Brm aa. 1400-1586
Clone 24-BRM
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Brm (SMARCA2 Antibody Abstract)
Background Several of the SNF and SWI genes of Saccharomyces cerevisiae encode proteins that are involved in the regulation of transcriptional activation. One of these proteins, SNF2/SWI2, has both Drosophila and mammalian homologues, designated brm. The human Brm protein has been reported to be a 180 kDa nuclear factor that acts as a transcriptional activator when fused to a heterologus DNA binding domain. Transfected Brm, expressed in cells lacking endogenous protein, can cooperate with the glucocorticoid receptor (GR) in transcriptional activation. The cooperation between Brm and GR requires the DNA binding domain of GR and the helicase domain and the P/Q-charged domain of Brm. However, Brm does not affect on several other transcription factors. The retinoblastoma protein, Rb, stimulates the transcription of a number of genes. Like Brm, Rb up-regulates glucocorticoid-receptor-mediated transcription. Brm and Rb interact in vitro and in vivo, requiring the Rb-pocket domain and the consensus Rb-binding motif of Brm. This antibody is routinely tested by western blot analysis.
Molecular Weight 180 kDa

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-SMARCA2 antibody (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2) (AA 1400-1586) (ABIN967938) Western blot analysis of Brm on a HeLa cell lysate (Human cervical epitheloid carcino...
Immunofluorescence (IF) image for anti-SMARCA2 antibody (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2) (AA 1400-1586) (ABIN967938) Immunofluorescence staining of a rabbit lung section.
Product cited in: Wong, Shanahan, Chen et al.: "BRG1, a component of the SWI-SNF complex, is mutated in multiple human tumor cell lines." in: Cancer research, Vol. 60, Issue 21, pp. 6171-7, 2000 (PubMed).

Muchardt, Yaniv: "A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor." in: The EMBO journal, Vol. 12, Issue 11, pp. 4279-90, 1993 (PubMed).

Singh, Coe, Hong: "A role for retinoblastoma protein in potentiating transcriptional activation by the glucocorticoid receptor." in: Nature, Vol. 374, Issue 6522, pp. 562-5, 1995 (PubMed).

Catalog No. ABIN967938
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