Signal Transducer and Activator of Transcription 4 (STAT4) (AA 136-258) antibody

Antigen: Signal Transducer and Activator of Transcription 4 (STAT4)

Synonyms: SLEB11, STAT4, MGC157175

Binding Site: AA 136-258

Clonality: Monoclonal (8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968254

Additional Information

Alternative name: Stat4

Immunogen: Human Stat4

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 8

Description: The Stat proteins function as both cytoplasmic signal transducers and activators of transcription. Stat91/84 (the two proteins are the result of alternate splicing-Stat91 has an additional 38 C-terminal amino acids) and Stat113 were the first identified members of this protein family. With the discovery of additional members of the Stat family (Stat 3 & 4), the nomenclature has been revised to reflect the order of their discovery. Stat 91, 84, and 113 have become Stat1alpha, Stat1beta, and Stat2, respectively. Stat4 has been reported to be 52% identical to Stat1 and 47% identical to Stat3. Functionally, Stat4 is similar to the other Stat proteins in that it requires tyrosine phosphorylation in order to stimulate its IFN-gamma activated site (GAS) binding activity. Expression of Stat4 appears to be limited to thymus, spleen, and testis. Its expression in several myeloid cell lines is differentially regulated during cellular differentiation. Co-transfection studies using JAK1 or JAK2 expression constructs indicate that Stat4 is a substrate for these kinases.
Synonyms: Signal Transducers and Activators of Transcription-4

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 89 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling, Receptors, Inflammation

Restrictions: For Research Use only

Images

Western blot analysis of Stat4 on a rat testis lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Stat4 antibody.

anti-Signal Transducer and Activator of Transcription 4 (STAT4) (AA 136-258) antibody (Image 2)

Publications

Product: Frank, Robertson, Bonni et al.: "Interleukin 2 signaling involves the phosphorylation of Stat proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 17, pp. 7779-83, 1995 (PubMed).

Zhong, Wen, Darnell: "Stat3 and Stat4: members of the family of signal transducers and activators of transcription." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 11, pp. 4806-10, 1994 (PubMed).

Yamamoto, Quelle, Thierfelder et al.: "Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation." in: Molecular and cellular biology, Vol. 14, Issue 7, pp. 4342-9, 1994 (PubMed).