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Details for Product No. ABIN968333

PARP (AA 22-219) antibody

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»Alternatives AA 22-219
»Alternatives Human
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
»Alternatives Un-conjugated
»Alternatives Western Blotting (WB), Immunofluorescence (IF)
Pubmed 5 references available
Catalog no. ABIN968333
Quantity 150 µg
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Immunogen Human PARP
Clone BF12
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Background Poly(ADP-ribose) polymerase (PARP) is a constitutively expressed, abundant nuclear protein. It has been referred to as a molecular nick sensor due to its recognition of, and catalytic activation by, single or double strand DNA breaks. The most critical ad extensively studied role of PARP is its participation in DNA base excision repair. Following binding to damaged DNA, PARP uses NAD+ to synthesize branched polymers of poly(ADP-ribose) on nuclear target proteins, including itself. Such modification of PARP increases its negative charge and results in loss of interactionwith DNA due to electrostatic repulsion. This opens the damaged DNA to DNA repair proteins. The poly(ADP-ribose) molecule is quickly degraded by poly (ADP-ribose) glycohydrolase that is found in association with PARP. PARP contains N-terminal DNA-binding domain (DBD), a central automodification domain that accepts poly (ADP-ribose), and a C-terminal catalytic domain. PARP is one of the earliest proteins targeted by caspase-3 during apoptosis. Although this protein is central to DNA repair, it has additional DNA-related functions that remain to be investigated. This antibody is routinely tested by western blot analysis.
Molecular Weight 113 kDa
Research Area Chromatin and Nuclear Signaling, DNA/RNA, Enzymes, Metabolism

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Product cited in: Cherney, McBride, Chen et al.: "cDNA sequence, protein structure, and chromosomal location of the human gene for poly(ADP-ribose) polymerase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 23, pp. 8370-4, 1988 (PubMed).

Duriez, Shah: "Cleavage of poly(ADP-ribose) polymerase: a sensitive parameter to study cell death." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 75, Issue 4, pp. 337-49, 1998 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).

Liao, Chien, Shenoy et al.: "Inhibition of constitutively active forms of mutant kit by multitargeted indolinone tyrosine kinase inhibitors." in: Blood, Vol. 100, Issue 2, pp. 585-93, 2002 (PubMed).

Jaiswal, Marlow, Gupta et al.: "Beta-catenin-mediated transactivation and cell-cell adhesion pathways are important in curcumin (diferuylmethane)-induced growth arrest and apoptosis in colon cancer cells." in: Oncogene, Vol. 21, Issue 55, pp. 8414-27, 2002 (PubMed).

Alternatives for antigen "PARP", type "Antibodies"
Hosts (36), (8)
Reactivities (41), (25), (19), (13), (12), (1), (1), (1), (1), (1), (1)
Applications (33), (17), (10), (8), (7), (4), (3), (3), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (5), (4), (4), (4), (2), (2), (2), (1), (1)