Itchy E3 Ubiquitin Protein Ligase Homolog (Mouse) (ITCH) (AA 114-220) antibody

Antigen: Itchy E3 Ubiquitin Protein Ligase Homolog (Mouse) (ITCH)

Synonyms: AIF4, AIP4, NAPP1, dJ468O1.1

Binding Site: AA 114-220

Clonality: Monoclonal (32)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968414

Additional Information

Alternative name: Itch

Immunogen: Mouse Itch

Cross-Reactivity: Rat (Rattus), Human, Rabbit

Format: Liquid

Isotype: IgG1

Clone: 32

Description: Maintenance of cellular function requires timely and selective degradation of key regulatory proteins. For example, progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins via the ubiquitin pathway. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an epsilon-NH2-Lys in a target protein. Ubiquitin-conjugated proteins are recognized and degraded by the 26S proteasome. Ubiquitination requires ubiquitin-activating enzyme E1, ubiquitin-conjugating enzymes E2, and ubiquitin ligases E3. The direction of ubiquitin transfer is from E1 to E2 and from E2 to E3. Itch, a novel E3 ubiquitin ligase, is absent in the Non-agouti-lethal 18H mice. These mice develop immunological, inflammatory, epithelial, and hematopoietic diseases. Itch contains four WW protein interaction domains, which bind to proline-rich sequences in a fashion similar to SH3 domains. In addition, Itch contains a C-terminal Hect domain, which is conserved in the E3 family of ubiquitin ligases. Thus, Itch is important in the ubiquitin-dependent protein degradation occurring in normal hematopoiesis and inflammation.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 113 kDa

Comments:

Related Products: ABIN968548, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Itch on rat liver lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-Itch antibody.

Immunofluorescent staining of HeLa cells with anti-Itch antibody.

Publications

Product: Perry, Hustad, Swing et al.: "The itchy locus encodes a novel ubiquitin protein ligase that is disrupted in a18H mice." in: Nature genetics, Vol. 18, Issue 2, pp. 143-6, 1998 (PubMed).