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ITCH antibody (Internal Region)

Verified ITCH Reactivity: Human ELISA, WB, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN334426
  • Target See all ITCH Antibodies
    ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
    Binding Specificity
    • 10
    • 8
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 37
    • 34
    • 8
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 46
    • 3
    Goat
    Clonality
    • 45
    • 4
    Polyclonal
    Conjugate
    • 27
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ITCH antibody is un-conjugated
    Application
    • 34
    • 32
    • 7
    • 3
    • 3
    • 1
    ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    ITCH / AIF4
    Sequence
    EIKSHDLKPN GGN
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.
    Isotype
    IgG
  • Application Notes

    Western Blot: Approx 90 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 86.4 kDa according to NP_001244067.1) . Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
    Alternative Name
    ITCH (ITCH Products)
    Synonyms
    AIF4 antibody, AIP4 antibody, NAPP1 antibody, dJ468O1.1 antibody, 6720481N21Rik antibody, 8030492O04Rik antibody, A130065M08 antibody, C230047C07Rik antibody, itchy E3 ubiquitin protein ligase antibody, itchy, E3 ubiquitin protein ligase antibody, ITCH antibody, Itch antibody
    Background
    ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse) , AIP4, NAPP1, dJ468O1.1 , NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiqui
    Gene ID
    83737, 16396, 311567
    NCBI Accession
    NP_113671, NP_001244066, NP_001244067
    Pathways
    Activation of Innate immune Response, CXCR4-mediated Signaling Events
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