MutS Homolog 3 (E. Coli) (MSH3) (AA 136-349) antibody

Antigen: MutS Homolog 3 (E. Coli) (MSH3)

Synonyms: DUP, MRP1, MGC163306, MGC163308, Rep3, Rep-3, D13Em1, MGC152093, DKFZp469H2415

Binding Site: AA 136-349

Clonality: Monoclonal (52)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968513

Additional Information

Alternative name: MSH3

Immunogen: Human MSH3

Format: Liquid

Isotype: IgG1

Clone: 52

Description: Bacterial mismatch DNA repair involves the MutL, MutH, and MutS proteins, which forms a complex that mediates excision repair. Mutations in or deficiencies of any of these proteins results in a mutator phenotype that is characterized by genetic instability. Human homologs of MutS include MSH2, MSH3, and MSH6. MSH2 forms heterodimers with MSH6 (hMutSalpha) or MSH3 (hMutSbeta) that specifically bind single-mispaired nucleotides and a subset of insertion-deletion mismatches. In addition, these heterodimers have intrinsic ATPase activity that is regulated by mismatch binding. ADP-bound heterodimers bind mismatched nucleotides, while ATP-bound heterodimers do not. The role of MSH3 in genetic stability in human cells in unclear. However, MSH3 and MSH6 share roles in the control of mutation rates. Both participate in repair of replication errors containing base-base mismatches or 1-4 extra bases. The MSH3 gene is located upstream of the dihydrofolate reductase (DHFR) gene and is expressed at low levels in a variety of human tissues. Thus, MSH3 is a component of an adenosine nucleotide-regulated molecular switch whose activity is essential for classical nucleotide mismatch repair.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Molecular Weight: 127 kDa

Comments:

Related Products: ABIN967389, ABIN968535

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis of MSH3 on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2). 2 µg/mL (lane 1), 1 µg/mL (lane 2) and 0.5 µg/mL (lane 3) of the mouse anti-human MSH3 antibody were used.

Immunofluorescence staining of human fibroblasts.

anti-MutS Homolog 3 (E. Coli) (MSH3) (AA 136-349) antibody (Image 3)

Publications

Product: New, Liu, Crouse: "The yeast gene MSH3 defines a new class of eukaryotic MutS homologues." in: Molecular & general genetics : MGG, Vol. 239, Issue 1-2, pp. 97-108, 1993 (PubMed).

Watanabe, Ikejima, Suzuki et al.: "Genomic organization and expression of the human MSH3 gene." in: Genomics, Vol. 31, Issue 3, pp. 311-8, 1997 (PubMed).

Umar, Risinger, Glaab et al.: "Functional overlap in mismatch repair by human MSH3 and MSH6." in: Genetics, Vol. 148, Issue 4, pp. 1637-46, 1998 (PubMed).

Wilson, Guerrette, Fishel: "Dissociation of mismatch recognition and ATPase activity by hMSH2-hMSH3." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21659-64, 1999 (PubMed).