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MSH3 antibody (AA 136-349)

MSH3 Reactivity: Human WB, IF Host: Mouse Monoclonal 52-MSH3 unconjugated
Catalog No. ABIN968513
  • Target See all MSH3 Antibodies
    MSH3 (MutS Homolog 3 (MSH3))
    Binding Specificity
    • 15
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 136-349
    Reactivity
    • 29
    • 3
    • 2
    Human
    Host
    • 29
    Mouse
    Clonality
    • 28
    • 1
    Monoclonal
    Conjugate
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MSH3 antibody is un-conjugated
    Application
    • 13
    • 13
    • 13
    • 9
    • 7
    • 4
    • 4
    • 4
    • 4
    • 2
    Western Blotting (WB), Immunofluorescence (IF)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Please refer to us for technical protocols.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human MSH3 aa. 136-349
    Clone
    52-MSH3
    Isotype
    IgG1
    Top Product
    Discover our top product MSH3 Primary Antibody
  • Comment

    Related Products: ABIN967389, ABIN968535

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Wilson, Guerrette, Fishel: "Dissociation of mismatch recognition and ATPase activity by hMSH2-hMSH3." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21659-64, (1999) (PubMed).

    Umar, Risinger, Glaab, Tindall, Barrett, Kunkel: "Functional overlap in mismatch repair by human MSH3 and MSH6." in: Genetics, Vol. 148, Issue 4, pp. 1637-46, (1998) (PubMed).

    Watanabe, Ikejima, Suzuki, Shimada: "Genomic organization and expression of the human MSH3 gene." in: Genomics, Vol. 31, Issue 3, pp. 311-8, (1997) (PubMed).

    New, Liu, Crouse: "The yeast gene MSH3 defines a new class of eukaryotic MutS homologues." in: Molecular & general genetics : MGG, Vol. 239, Issue 1-2, pp. 97-108, (1993) (PubMed).

  • Target
    MSH3 (MutS Homolog 3 (MSH3))
    Alternative Name
    MSH3 (MSH3 Products)
    Synonyms
    ATMSH3 antibody, M7J2.90 antibody, M7J2_90 antibody, homolog of DNA mismatch repair protein MSH3 antibody, DUP antibody, MRP1 antibody, D13Em1 antibody, Rep-3 antibody, Rep3 antibody, mutS homolog 3 antibody, DNA mismatch repair protein MSH3 antibody, homolog of DNA mismatch repair protein MSH3 antibody, mismatch repair protein antibody, MSH3 antibody, Msh3 antibody
    Background
    Bacterial mismatch DNA repair involves the MutL, MutH, and MutS proteins, which forms a complex that mediates excision repair. Mutations in or deficiencies of any of these proteins results in a mutator phenotype that is characterized by genetic instability. Human homologs of MutS include MSH2, MSH3, and MSH6. MSH2 forms heterodimers with MSH6 (hMutSalpha) or MSH3 (hMutSbeta) that specifically bind single-mispaired nucleotides and a subset of insertion-deletion mismatches. In addition, these heterodimers have intrinsic ATPase activity that is regulated by mismatch binding. ADP-bound heterodimers bind mismatched nucleotides, while ATP-bound heterodimers do not. The role of MSH3 in genetic stability in human cells in unclear. However, MSH3 and MSH6 share roles in the control of mutation rates. Both participate in repair of replication errors containing base-base mismatches or 1-4 extra bases. The MSH3 gene is located upstream of the dihydrofolate reductase (DHFR) gene and is expressed at low levels in a variety of human tissues. Thus, MSH3 is a component of an adenosine nucleotide-regulated molecular switch whose activity is essential for classical nucleotide mismatch repair.
    Molecular Weight
    127 kDa
    Pathways
    DNA Damage Repair, Production of Molecular Mediator of Immune Response
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