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PCNT antibody (AA 1692-1814)

PCNT Reactivity: Mouse WB, IF Host: Mouse Monoclonal 30-Pericentrin unconjugated
Catalog No. ABIN968666
  • Target See all PCNT Antibodies
    PCNT (Pericentrin (PCNT))
    Binding Specificity
    • 4
    • 3
    • 1
    • 1
    • 1
    AA 1692-1814
    Reactivity
    • 11
    • 2
    • 1
    Mouse
    Host
    • 12
    Mouse
    Clonality
    • 12
    Monoclonal
    Conjugate
    • 9
    • 1
    • 1
    • 1
    This PCNT antibody is un-conjugated
    Application
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Mouse pericentrin aa. 1692-1814
    Clone
    30-Pericentrin
    Isotype
    IgG1
    Top Product
    Discover our top product PCNT Primary Antibody
  • Comment

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Arellano, Guadiana, Breunig, Rakic, Sarkisian: "Development and distribution of neuronal cilia in mouse neocortex." in: The Journal of comparative neurology, Vol. 520, Issue 4, pp. 848-73, (2012) (PubMed).

    Young, Dictenberg, Purohit, Tuft, Doxsey: "Cytoplasmic dynein-mediated assembly of pericentrin and gamma tubulin onto centrosomes." in: Molecular biology of the cell, Vol. 11, Issue 6, pp. 2047-56, (2000) (PubMed).

    Doxsey, Stein, Evans, Calarco, Kirschner: "Pericentrin, a highly conserved centrosome protein involved in microtubule organization." in: Cell, Vol. 76, Issue 4, pp. 639-50, (1994) (PubMed).

  • Target
    PCNT (Pericentrin (PCNT))
    Alternative Name
    Pericentrin (PCNT Products)
    Synonyms
    AKAP450 antibody, CG13459 antibody, CG18648 antibody, CG33957 antibody, CG6735 antibody, CP309 antibody, Cp309 antibody, D-PLP antibody, DPLP antibody, Dmel\\CG33957 antibody, Dplp antibody, PACT antibody, PLP antibody, Plp antibody, d-plp antibody, dPLP antibody, dplp antibody, l(3)s2172 antibody, pPlp antibody, KEN antibody, MOPD2 antibody, PCN antibody, PCNT2 antibody, PCNTB antibody, PCTN2 antibody, SCKL4 antibody, DIP2A antibody, AW476095 antibody, C86676 antibody, Pcnt2 antibody, kendrin antibody, m239Asp antibody, m275Asp antibody, pericentrin antibody, Pericentrin-like protein antibody, pericentrin (kendrin) antibody, LOC100055203 antibody, Plp antibody, PCNT antibody, pcnt antibody, Pcnt antibody
    Background
    Centrosomes and other microtubule-organizing centers are a diverse group of organelles that nucleate and organize microtubules for many celllular processes, such as mitotic spindle formation, organelle tranpsort, and protein localization. Pericentrin is a protein in the pericentriolar material, a filamentous matrix surrounding centrioles, which can organize microtubule spindle formation. The structure of pericentrin includes multiple alpha-helical domains that form coiled-coil domains separated by non-helical, non-coiled regions. It is found in a 3 MDa-complex that includes gamma-tubulin, a form of tubulin that nucleates microtubule formation at the centrosome. Pericentrin exhibits the highest expression in embryonic mouse kidney, thymus, and liver. Injection of pericentrin antibodies in Xenopus oocytes disrupts mitotic and meiotic divisions and blocks microtubule aster formation. In addition, dynein transports both pericentrin and gamma-tubulin to centrosomes along microtubules in Xenopus oocyte extracts. Thus, the complex of pericentrin and gamma-tubulin may be recruited to the centrosome by dynein, where the complex becomes anchored to the centrosome for microtubule nucleating activity. This antibody is routinely tested by western blot analysis.
    Molecular Weight
    220 kDa
    Pathways
    Sensory Perception of Sound, M Phase, SARS-CoV-2 Protein Interactome
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